PXD005516

PXD005516 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Analysis of Paper Mulberry under Chilling
Description	Paper mulberry as a valuable woody species has a well chilling tolerance. In this study, phosphoproteomic analysis in combination with physiological measurement and mRNA quantification were employed to explore the molecular mechanism of chilling (4 °C) tolerance in paper mulberry. After chilling for 6 hours, there were 427 significant changed phosphoproteins detected in paper mulberry seedlings without obvious physiological injury. When obvious physiological injury occurred after chilling for 48 hours, a total of 611 phosphoproteins were found significantly change at phosphorylation level. According to 9 phosphorylation motifs extracted by Motif-X analysis, MAPKs, CDPKs, CDKs and CKs were considered as the primary upstream protein kinases. Results of GO analysis showed that phosphoproteins were mainly responsible for signal transduction, protein modification and translation during chilling. Additionally, transport and cellular component organization were respectively enriched after chilling for 6 and 48 hours. Based on the analysis of protein-protein interaction network, a protein kinases and phosphatases hub protein was thought as the key of phosphorylation regulation, which probably modulates cross-talk between Ca2+, BR, ABA and ethylene mediated signaling pathways. Together with results, we concluded a schematic chilling tolerance mechanism at phosphorylation level.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:31:33.421.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhi Pi
SpeciesList	scientific name: Broussonetia papyrifera; NCBI TaxID: 172644;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-07 01:50:20	ID requested
1	2018-04-20 07:10:35	announced
⏵ 2	2024-10-22 04:31:34	announced	2024-10-22: Updated project metadata.

Pi Z, Zhao ML, Peng XJ, Shen SH, Phosphoproteomic Analysis of Paper Mulberry Reveals Phosphorylation Functions in Chilling Tolerance. J Proteome Res, 16(5):1944-1961(2017) [pubmed]

10.1021/acs.jproteome.6b01016;

curator keyword: Biological

submitter keyword: chilling tolerance, phosphorylation, paper mulberry

Shen lab
contact affiliation	Key Laboratory of Plant Resources, Institute of Botany, the Chinese Academy of Sciences, Beijing
contact email	pizhi@ibcas.ac.cn
lab head
Zhi Pi
contact affiliation	Institute of Botany, the Chinese Academy of Sciences
contact email	pizhi@ibcas.ac.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005516
     1. Label: PRIDE project
     2. Name: Phosphoproteomic Analysis of Paper Mulberry under Chilling