PXD005507

PXD005507 is an original dataset announced via ProteomeXchange.

Title	Structurally related hydrazone-based metal complexes with different antitumor activities variably induce apoptotic cell death
Description	Three new complexes bearing the tridentate hydrazone-based ligand 2-(2-(1-(pyridin-2-yl)ethylidene)hydrazinyl)pyridine (L) were synthesized and structurally characterized. Biological tests indicate that the Zn(II) complex [ZnCl2(L)] • 0.5 H2O is of low cytotoxicity against the hepatocellular carcinoma cell line HepG2. In contrast, the Cu(II) and Mn(II) complexes [CuCl2(L)] and [MnCl2(L)(OH2)] • H2O are highly cytotoxic with EC50 values of 1.25  0.01 M and 20  1 M, respectively. A quantitative proteome analysis reveals that treatment of the cells with the Cu(II) complex leads to a significantly altered abundance of 102 apoptosis-related proteins, whereas 38 proteins were up- or down-regulated by the Mn(II) complex. A closer inspection of those proteins regulated only by the Cu(II) complex suggests that the superior cytotoxic activity of this complex is likely to be related to an initiation of caspase-independent cell death (CICD).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:34:10.542.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005507
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dominik Andre Meger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-06 03:50:18	ID requested
1	2017-05-03 08:48:02	announced
⏵ 2	2024-10-22 04:34:11	announced	2024-10-22: Updated project metadata.

10.1039/c6dt04613d;

Megger DA, Rosowski K, Radunsky C, K, ö, sters J, Sitek B, M, ü, ller J, Structurally related hydrazone-based metal complexes with different antitumor activities variably induce apoptotic cell death. Dalton Trans, 46(14):4759-4767(2017) [pubmed]

10.6019/PXD005507;

curator keyword: Biological, Biomedical

submitter keyword: LC-MS/MS, Metal Complexes, Liver Cancer

Dominik A. Megger
contact affiliation	Medizinisches Proteom-Center Ruhr-Universität Bochum Universitätsstr. 150 44801 Bochum Germany
contact email	dominik.megger@rub.de
lab head
Dominik Andre Meger
contact affiliation	Medizinisches Proteom-Center (MPC), Ruhr-Universität Bochum, Germany
contact email	dominik.megger@rub.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD005507

PRIDE project URI

• PRIDE
  1. PXD005507
     1. Label: PRIDE project
     2. Name: Structurally related hydrazone-based metal complexes with different antitumor activities variably induce apoptotic cell death