PXD005503

PXD005503 is an original dataset announced via ProteomeXchange.

Title	N-glycosylated Proteins of Cryptosporidium parvum
Description	Here, we used mass spectrometry to identify the glycoproteins, localize the position of modification, and determine in detail the structures of the N-glycans. Using whole oocyst lysates we identified the most abundant N-glycosylated proteins, including some of the previously identified immunodominant antigens.
HostingRepository	PRIDE
AnnounceDate	2017-02-10
AnnouncementXML	Submission_2017-02-10_02:58:45.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005503
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	John Haserick
SpeciesList	scientific name: Cryptosporidium parvum; NCBI TaxID: 5807;
ModificationList	Dethiomethyl; Amidated; Diethyl; Bacillosamine; Hex; Pro->pyro-Glu; Phospho; Deamidated; sulfo+amino; Trp->Oxolactone; Acetyl; Cation:Ni[II]; Carboxymethyl; HexNAc; Hex1HexNAc1; Carbamidomethyl; Cation:Ca[II]; Gln->pyro-Glu; Diethylphosphate; Guanidinyl; Menadione-HQ; Glu->pyro-Glu; DAET; MercaptoEthanol; Ethyl; Ammonia-loss; Cation:Fe[II]; Methyl; AEBS; Oxidation; ISD_z+2_ion; AEC-MAEC; Ethanedithiol; Carboxy; Didehydro; Cation:Na; Hex(2)HexNAc(2); SMA; GlyGly; BHAc; Hex(5)HexNAc(2); HexN; Diisopropylphosphate; Methyl+Deamidated; Nmethylmaleimide; Formyl; Carbamyl; maleimide; Dehydrated; Ammonium; Biotin; Dimethyl; Dioxidation; Carbofuran
Instrument	LTQ Orbitrap; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-05 06:06:47	ID requested
⏵ 1	2017-02-10 02:58:46	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: N-glycosylation, LC-MS/MS, cryptosporidiosis, Cryptosporidium parvum

Catherine E. Costello
contact affiliation	Center for Biomedical Mass Spectrometry, Department of Biochemistry, Boston University School of Medicine, Boston, Massachusetts, USA
contact email	cecmsms@bu.edu
lab head
John Haserick
contact affiliation	Boston University
contact email	haserick@bu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005503

PRIDE project URI

• PRIDE
  1. PXD005503
     1. Label: PRIDE project
     2. Name: N-glycosylated Proteins of Cryptosporidium parvum