PXD005500

PXD005500 is an original dataset announced via ProteomeXchange.

Title	Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin enzymes
Description	Protein ubiquitination is mediated sequentially by ubiquitin activating enzyme E1, ubiquitin conjugating enzyme E2, and ubiquitin ligase E3. Uba1 was thought to be the only E1 until the recent identification of Uba6 as an alternative. To differentiate the biological functions of Uba1 and Uba6, we applied a novel orthogonal ubiquitin transfer (OUT) technology to profile their ubiquitination targets in mammalian cells. By expressing pairs of an engineered ubiquitin and engineered Uba1 or Uba6 that were generated for exclusive interactions, we identified 697 Uba6 targets and 529 Uba1 targets with 258 overlaps. Bioinformatics revealed substantial differences in pathways involving Uba1- and Uba6-specific targets. We demonstrated that polyubiquitination and proteasomal degradation of ezrin and CUGBP1 require Uba6, but not Uba1, and Uba6 is involved in the control of ezrin localization and epithelial morphogenesis. These data reveal the distinctive substrate pools for Uba1 and Uba6 and manifest non-redundant biological roles for Uba6
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_12:21:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dhaval Nanavati
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-05 02:03:46	ID requested
⏵ 1	2018-10-26 12:21:54	announced

Liu X, Zhao B, Sun L, Bhuripanyo K, Wang Y, Bi Y, Davuluri RV, Duong DM, Nanavati D, Yin J, Kiyokawa H, Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin activating enzymes. Nat Commun, 8():14286(2017) [pubmed]

curator keyword: Biological

submitter keyword: Ubquitin, LC/MS,

Professor Hiroaki Kiyokawa
contact affiliation	Professor of Pharmacology and Pathology Northwestern Univeristy, Feinberg School of Medcine, Ward Building Room 9-176 303 E Chicago Avenue Chicago IL 60611
contact email	kiyokawa@northwestern.edu
lab head
Dhaval Nanavati
contact affiliation	Abbvie
contact email	dhaval.nanavati@abbvie.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005500
     1. Label: PRIDE project
     2. Name: Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin enzymes