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PXD005493

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-12-06
  • AnnouncementXML: Submission_2017-12-06_10:07:46.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Francois-Michel Boisvert
  • Title: Interactome of NudCD1 isoforms 1, 2 & 3
  • Description: NudCD1, also known as CML66 or OVA66, is a 583 amino acids protein initially identified as overexpressed in patients with chronic myelogenous leukemia. The mRNA of NudCD1 is expressed in heart and testis of normal tissues, and is overexpressed in several cancers. Previous studies have shown that the expression level of the protein correlates with tumoral phenotype, possibly interacting upstream of the Insulin Growth Factor - 1 Receptor (IGF-1R). The gene encoding the NudCD1 protein consists of 12 exons that can be alternative spliced, leading to the expression of three different isoforms. These isoforms possess a common region of 492 amino acids in their C-terminus region and have an isoform specific N-terminus. To determine the distinct function of each isoforms, we have localised the isoforms within the cells using immunofluorescence microscopy and used a quantitative proteomics approach (SILAC) to identify specific protein interaction partners for each isoforms. Location studies showed a different cellular location for the different isoforms, with the first isoform being nuclear, while the other two isoforms have distinct cytoplasmic and nuclear location. We found that the different NudCD1 isoforms have unique interacting partners, with the first isoform binding to a putative RNA helicase named DHX15 possibly involved in mRNA splicing.
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: 6x(13)C labeled residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-12-03 08:16:21ID requested
12017-12-06 10:07:47announced

Publication List

  1. Asselin-Mullen P, Chauvin A, Dubois ML, Drissi R, Lévesque D, Boisvert FM, Protein interaction network of alternatively spliced NudCD1 isoforms. Sci Rep, 7(1):12987(2017) [pubmed]

Keyword List

  1. curator keyword: Biological, Biomedical
  2. submitter keyword: Human, NudCD1, mRNA splicing, cancer

Contact List

    Francois-Michel Boisvert
    • contact affiliation: Université de Sherbrooke
    • contact email: fm.boisvert@usherbrooke.ca
    • lab head:
    Francois-Michel Boisvert
    • contact affiliation: Université de Sherbrooke
    • contact email: fm.boisvert@usherbrooke.ca
    • dataset submitter:

Full Dataset Link List

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Repository Record List
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