PXD005493

PXD005493 is an original dataset announced via ProteomeXchange.

Title	Interactome of NudCD1 isoforms 1, 2 & 3
Description	NudCD1, also known as CML66 or OVA66, is a 583 amino acids protein initially identified as overexpressed in patients with chronic myelogenous leukemia. The mRNA of NudCD1 is expressed in heart and testis of normal tissues, and is overexpressed in several cancers. Previous studies have shown that the expression level of the protein correlates with tumoral phenotype, possibly interacting upstream of the Insulin Growth Factor - 1 Receptor (IGF-1R). The gene encoding the NudCD1 protein consists of 12 exons that can be alternative spliced, leading to the expression of three different isoforms. These isoforms possess a common region of 492 amino acids in their C-terminus region and have an isoform specific N-terminus. To determine the distinct function of each isoforms, we have localised the isoforms within the cells using immunofluorescence microscopy and used a quantitative proteomics approach (SILAC) to identify specific protein interaction partners for each isoforms. Location studies showed a different cellular location for the different isoforms, with the first isoform being nuclear, while the other two isoforms have distinct cytoplasmic and nuclear location. We found that the different NudCD1 isoforms have unique interacting partners, with the first isoform binding to a putative RNA helicase named DHX15 possibly involved in mRNA splicing.
HostingRepository	PRIDE
AnnounceDate	2017-12-06
AnnouncementXML	Submission_2017-12-06_10:07:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Francois-Michel Boisvert
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	6x(13)C labeled residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-03 08:16:21	ID requested
⏵ 1	2017-12-06 10:07:47	announced

Asselin-Mullen P, Chauvin A, Dubois ML, Drissi R, L, é, vesque D, Boisvert FM, Protein interaction network of alternatively spliced NudCD1 isoforms. Sci Rep, 7(1):12987(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, NudCD1, mRNA splicing, cancer

Francois-Michel Boisvert
contact affiliation	Université de Sherbrooke
contact email	fm.boisvert@usherbrooke.ca
lab head
Francois-Michel Boisvert
contact affiliation	Université de Sherbrooke
contact email	fm.boisvert@usherbrooke.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005493
     1. Label: PRIDE project
     2. Name: Interactome of NudCD1 isoforms 1, 2 & 3