PXD005492

PXD005492 is an original dataset announced via ProteomeXchange.

Title	Integrative proteomics and phosphoproteomics profiling and systems biology analysis reveal dynamic signaling networks and mTORC1-dependent mitochondrial function in T cell activation
Description	The underlying mechanisms by which naïve T cells exit from quiescence after antigen stimulation remain elusive. Using multiplex isobaric labeling proteomics technology, we report unbiased, temporal profiling of whole proteome and phosphoproteome during the activation in the wild type and Rptor-/- T cells. TCR stimulation results in dynamic reprogramming of the proteome and phosphoproteome, with predominant upregulation of molecular machineries in protein translation and mitochondrial activation. Loss of mTORC1 disrupts TCR-induced mitochondrial functions including mitoribosome biogenesis, one-carbon metabolism and oxidative phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2017-03-29
AnnouncementXML	Submission_2017-03-29_00:17:32.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	xusheng wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-12-03 08:10:24	ID requested
⏵ 1	2017-03-29 00:17:33	announced

Tan H, Yang K, Li Y, Shaw TI, Wang Y, Blanco DB, Wang X, Cho JH, Wang H, Rankin S, Guy C, Peng J, Chi H, Integrative Proteomics and Phosphoproteomics Profiling Reveals Dynamic Signaling Networks and Bioenergetics Pathways Underlying T Cell Activation. Immunity, 46(3):488-503(2017) [pubmed]

curator keyword: Biological

submitter keyword: T cell activation, proteomic, phosphoproteomics, mass spectrometry, system biology, mTORC1, mitochondrial function, Rptor

Junmin Peng, PhD
contact affiliation	Structural Biology MS 311, Room D1034C St. Jude Children's Research Hospital 262 Danny Thomas Place Memphis, TN 38105-3678
contact email	junmin.peng@stjude.org
lab head
xusheng wang
contact affiliation	Proteomics
contact email	xushengwang78@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005492
     1. Label: PRIDE project
     2. Name: Integrative proteomics and phosphoproteomics profiling and systems biology analysis reveal dynamic signaling networks and mTORC1-dependent mitochondrial function in T cell activation