<<< Full experiment listing

PXD005474

PXD005474 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic analysis of pemphigus autoantibodies indicates a much larger, more diverse, and dynamic repertoire than determined by B-cell genetics
DescriptionGenetic techniques such as antibody phage display have indicated an oligoclonal autoantibody response in various autoimmune diseases including pemphigus. These techniques have limited sampling of B cell clones. Characterization by mass spectometry of pemphigus serum autoantibodies affinity-purified on the autoantigen desmoglein indicates a much more polyclonal response. Conversely, many genetically detectable anti-desmoglein B cells do not contribute detectably to the serum antibody response. There is no convergence of the autoantibody response among patients, as determined by CDR3 sequence or heavy chain variable gene usage, implying targeting of these genes will not be a useful therapeutic strategy. Longitudinal analysis of autoantibodies over years indicates that, although many antibody clones persist, the proportion of each clonal antibody changes. These studies indicate a dynamic and very diverse autoantibody response not revealed by genetic studies, and explain why similar overall anti-desmoglein titers may give variable disease activity.
HostingRepositoryMassIVE
AnnounceDate2018-05-24
AnnouncementXMLSubmission_2018-05-24_09:29:49.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHsin-Yao Tang
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; Deamidated
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-11-30 11:21:09ID requested
12018-05-24 09:29:50announced
Publication List
no publication
Keyword List
submitter keyword: Pemphigus, autoantibody, LC-MS/MS, Proteomics
Contact List
John R. Stanley
lab head
Hsin-Yao Tang
contact affiliationThe Wistar Institute
contact emailtangh@wistar.org
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location