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DataSet Summary

  • HostingRepository: MassIVE
  • AnnounceDate: 2018-05-24
  • AnnouncementXML: Submission_2018-05-24_09:29:49.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Non peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Hsin-Yao Tang
  • Title: Proteomic analysis of pemphigus autoantibodies indicates a much larger, more diverse, and dynamic repertoire than determined by B-cell genetics
  • Description: Genetic techniques such as antibody phage display have indicated an oligoclonal autoantibody response in various autoimmune diseases including pemphigus. These techniques have limited sampling of B cell clones. Characterization by mass spectometry of pemphigus serum autoantibodies affinity-purified on the autoantigen desmoglein indicates a much more polyclonal response. Conversely, many genetically detectable anti-desmoglein B cells do not contribute detectably to the serum antibody response. There is no convergence of the autoantibody response among patients, as determined by CDR3 sequence or heavy chain variable gene usage, implying targeting of these genes will not be a useful therapeutic strategy. Longitudinal analysis of autoantibodies over years indicates that, although many antibody clones persist, the proportion of each clonal antibody changes. These studies indicate a dynamic and very diverse autoantibody response not revealed by genetic studies, and explain why similar overall anti-desmoglein titers may give variable disease activity.
  • SpeciesList: scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
  • ModificationList: Oxidation; Deamidated
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-11-30 11:21:09ID requested
12018-05-24 09:29:50announced

Publication List

  1. no publication

Keyword List

  1. submitter keyword: Pemphigus, autoantibody, LC-MS/MS, Proteomics

Contact List

    John R. Stanley
    • lab head:
    Hsin-Yao Tang
    • contact affiliation: The Wistar Institute
    • contact email: tangh@wistar.org
    • dataset submitter:

Full Dataset Link List

  1. MassIVE dataset URI
  2. Dataset FTP location

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