PXD005472

PXD005472 is an original dataset announced via ProteomeXchange.

Title	Na2CO3-responsive phosphoproteins in chloroplasts from Puccinellia tenuiflora leaves
Description	Alkali-salinity exerts severe osmotic, ionic, and high-pH stresses to plants. Photosynthetic machinery is especially sensitive to saline-alkali stress. In addition, reversible protein phosphorylation also play crucial roles in plant salt resistance. While our knowledge on protein phosphorylation events in salt stress response is very limited. Few studies have been published involving photosynthetic protein phosphorylation modulation to improve salt resistance. In the present study, we investigated the Na2CO3-responsive characteristics in Puccinellia tenuiflora chloroplasts using stable isotope dimethyl labeled phosphoproteomic approach. A total of 161 unique phosphopeptides were identified, and 137 phosphopeptides were quantified by dimethyl labeling. Among them, 50 proteins were quantified as Na2CO3-responsive proteins with 57 phosphosites, including 33 increased and 14 decreased. Importantly, 26 phosphosites were newly identified as Na2CO3-responsive phosphoproteins in plants, which were supposed to be crucial for regulating photosynthesis, membrane and transporting, signaling, stress response, and protein synthesis and turnover. Among them, seven light harvesting proteins, six PSII proteins, five PSI proteins, three electron transfer chain proteins, and two Calvin cycle-related proteins were increased, but five ATP synthase subunits and sucrose-phosphate synthase were decreased at phosphorylation level. Besides, Na+/H+ antiporter, villin-2, and two thylakoid organization related proteins were increased at phosphorylation level. Two signaling related proteins and most protein species in charge of gene expression and protein turnover were enhanced at phosphorylation level at 24h after Na2CO3 treatment.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:33:24.338.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaojun Dai
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-30 05:29:38	ID requested
1	2022-02-22 03:57:30	announced
⏵ 2	2024-10-22 05:33:25	announced	2024-10-22: Updated project metadata.

10.1016/j.gpb.2018.10.011;

Suo J, Zhang H, Zhao Q, Zhang N, Zhang Y, Li Y, Song B, Yu J, Cao J, Wang T, Luo J, Guo L, Ma J, Zhang X, She Y, Peng L, Ma W, Guo S, Miao Y, Chen S, Qin Z, Dai S, -responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics. Genomics Proteomics Bioinformatics, 18(3):271-288(2020) [pubmed]

submitter keyword: Na2CO3, Phosphoproteomics, Chloroplast, Puccinellia tenuiflora

Shaojun Dai
contact affiliation	Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China
contact email	735425617@qq.com
lab head
Shaojun Dai
contact affiliation	NEFU
contact email	735425617@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005472
     1. Label: PRIDE project
     2. Name: Na2CO3-responsive phosphoproteins in chloroplasts from Puccinellia tenuiflora leaves