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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-05-19
  • AnnouncementXML: Submission_2017-06-02_04:49:06.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Eugene Dillon
  • Title: SIN3A Acetylation Complex
  • Description: Sin3a is the central scaffold protein of the prototypical Hdac1/2 chromatin repressor complex, crucially required during early embryonic development for the growth of pluripotent cells of the inner cell mass. Here, we explore the endogenous composition of the Sin3a-Hdac complex in pluripotent embryonic stem (ES) and differentiated cells. To do this, we established an endogenous double immunoprecipitation strategy coupled with quantitative mass spectrometry (ENDIP-MS) allowing us to define the precise composition of the Sin3a complex in multiple cell types. We identify the Fam60a subunit as a key defining feature of a variant Sin3a complex present in ES cells, but not in differentiated cells. Fam60a co-occupies H3K4me3 positive promoters with Sin3a and is essential to maintain it on chromatin. Consistent with this, Fam60a depletion phenocopies the loss of Sin3a, leading to decreased proliferation, an extended G1-phase and the deregulation of genes associated with differentiation. Taken together, our data characterise Fam60a as an essential core subunit of a variant Sin3a complex in ES cells required to promote rapid proliferation and to prevent unscheduled differentiation.
  • SpeciesList: scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: monohydroxylated residue; acetylated residue; iodoacetic acid derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-11-30 01:24:43ID requested
12017-05-19 02:19:38announced
22017-06-02 04:49:07announcedUpdated publication reference for PubMed record(s): 28554894.

Publication List

  1. Streubel G, Fitzpatrick DJ, Oliviero G, Scelfo A, Moran B, Das S, Munawar N, Watson A, Wynne K, Negri GL, Dillon ET, Jammula S, Hokamp K, O'Connor DP, Pasini D, Cagney G, Bracken AP, Fam60a defines a variant Sin3a-Hdac complex in embryonic stem cells required for self-renewal. EMBO J, 36(15):2216-2232(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Fam60a, Sin3a complex, Embryonic stem cell, Self-renewal

Contact List

    Dr Gerard Cagney
    • contact affiliation: Group Leader School of Biomolecular and Biomedical Science Room F058, Conway Institute, University College Dublin, Belfield, Dublin 4, IRELAND
    • contact email: gerard.cagney@ucd.ie
    • lab head:
    Eugene Dillon
    • contact affiliation: UCD
    • contact email: eugene.dillon@ucd.ie
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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