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PXD005464

PXD005464 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSIN3A Acetylation Complex
DescriptionSin3a is the central scaffold protein of the prototypical Hdac1/2 chromatin repressor complex, crucially required during early embryonic development for the growth of pluripotent cells of the inner cell mass. Here, we explore the endogenous composition of the Sin3a-Hdac complex in pluripotent embryonic stem (ES) and differentiated cells. To do this, we established an endogenous double immunoprecipitation strategy coupled with quantitative mass spectrometry (ENDIP-MS) allowing us to define the precise composition of the Sin3a complex in multiple cell types. We identify the Fam60a subunit as a key defining feature of a variant Sin3a complex present in ES cells, but not in differentiated cells. Fam60a co-occupies H3K4me3 positive promoters with Sin3a and is essential to maintain it on chromatin. Consistent with this, Fam60a depletion phenocopies the loss of Sin3a, leading to decreased proliferation, an extended G1-phase and the deregulation of genes associated with differentiation. Taken together, our data characterise Fam60a as an essential core subunit of a variant Sin3a complex in ES cells required to promote rapid proliferation and to prevent unscheduled differentiation.
HostingRepositoryPRIDE
AnnounceDate2017-05-19
AnnouncementXMLSubmission_2017-06-02_04:49:06.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterEugene Dillon
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetic acid derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-11-30 01:24:43ID requested
12017-05-19 02:19:38announced
22017-06-02 04:49:07announcedUpdated publication reference for PubMed record(s): 28554894.
Publication List
Streubel G, Fitzpatrick DJ, Oliviero G, Scelfo A, Moran B, Das S, Munawar N, Watson A, Wynne K, Negri GL, Dillon ET, Jammula S, Hokamp K, O'Connor DP, Pasini D, Cagney G, Bracken AP, Fam60a defines a variant Sin3a-Hdac complex in embryonic stem cells required for self-renewal. EMBO J, 36(15):2216-2232(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Fam60a, Sin3a complex, Embryonic stem cell, Self-renewal
Contact List
Dr Gerard Cagney
contact affiliationGroup Leader School of Biomolecular and Biomedical Science Room F058, Conway Institute, University College Dublin, Belfield, Dublin 4, IRELAND
contact emailgerard.cagney@ucd.ie
lab head
Eugene Dillon
contact affiliationUCD
contact emaileugene.dillon@ucd.ie
dataset submitter
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Dataset FTP location
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