PXD005455

PXD005455 is an original dataset announced via ProteomeXchange.

Title	Na2CO3-responsive proteins in Puccinellia tenuiflora leaves
Description	Puccinellia tenuiflora is a monocotyledonous halophyte species belonging to the Gramineae. It has strong ability for surviving in the extreme saline-alkali soil (pH range of 9-10). In the present study, proteins from leaves under various Na2CO3 treatments were separated and visualized on Coomassie Brilliant Blue-stained two-dimensional gel electrophoresis (2-DE) gels. After gel image analysis on the base of the calculated average vol% values of each protein spot, 174 protein spots were detected as abundance changed protein spots (1.5-fold changes, p < 0.05). Among them, 104 protein spots were identified using MALDI-TOF MS/MS and Mascot database searching. Thus the 104 protein identities represented 80 unique proteins were taken as Na2CO3-responsive proteins in leaves. After integrative analysis by BLAST alignment, Kyoto Encyclopedia of Genes and Genomes, and referring information from related literature, they were classified into 10 functional categories, including photosynthesis, carbohydrate and energy metabolism, other metabolisms, stress and defense, membrane and transport, signaling, protein synthesis, folding, and turnover, cell wall mechanism, cell cycle, and miscellaneous or unknown.
HostingRepository	PRIDE
AnnounceDate	2021-08-30
AnnouncementXML	Submission_2021-08-30_02:05:50.277.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shaojun Dai
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-28 06:04:10	ID requested
⏵ 1	2021-08-30 02:05:51	announced

Suo J, Zhang H, Zhao Q, Zhang N, Zhang Y, Li Y, Song B, Yu J, Cao J, Wang T, Luo J, Guo L, Ma J, Zhang X, She Y, Peng L, Ma W, Guo S, Miao Y, Chen S, Qin Z, Dai S, -responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics. Genomics Proteomics Bioinformatics, 18(3):271-288(2020) [pubmed]

curator keyword: Biological

submitter keyword: Na2CO3, Puccinellia tenuiflora, Leaf, Proteomics

Shaojun Dai
contact affiliation	Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Ministry of Education, Harbin 150040, China
contact email	735425617@qq.com
lab head
Shaojun Dai
contact affiliation	NEFU
contact email	735425617@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005455
     1. Label: PRIDE project
     2. Name: Na2CO3-responsive proteins in Puccinellia tenuiflora leaves