PXD005453

PXD005453 is an original dataset announced via ProteomeXchange.

Title	Human ccRCC cell line 786-O with PHD3 knockdown LC-MS/MS
Description	Appreciating the pseudohypoxic signalling in ccRCC cells we decided to look into the effects of an established hypoxia-related enzyme, namely PHD3, in ccRCC development. Interestingly, these cells express PHD3 in unusually high amounts, a phenomena yet unexplained. We were interested in determining how ccRCC cells expressing elevated levels of PHD3 would respond to its knockdown. It has been shown that PHD3 has many other functions in addition to its given role as regulating HIF-1 by targeting it for proteosomal degradation. Motivated by these varied roles of PHD3 we decided to look for indications of a systemic-level changes that might be governed by PHD3 in ccRCC. To this end we chose a discovery proteomics –approach to see how ccRCC cells would respond on proteome level to PHD3 depletion.
HostingRepository	PRIDE
AnnounceDate	2017-07-10
AnnouncementXML	Submission_2017-07-10_00:48:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Petra Miikkulainen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-28 04:39:41	ID requested
⏵ 1	2017-07-10 00:48:02	announced

Miikkulainen P, H, ö, gel H, Rantanen K, Suomi T, Kouvonen P, Elo LL, Jaakkola PM, HIF prolyl hydroxylase PHD3 regulates translational machinery and glucose metabolism in clear cell renal cell carcinoma. Cancer Metab, 5():5(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Human, ccRCC, cancer, PHD3

Panu M. Jaakkola
contact affiliation	Department of Medical Biochemistry, Faculty of Medicine, University of Turku, FI-20520 Turku, Finland and Department of Oncology and Radiotherapy, Turku University Hospital, FI-20520 Turku, Finland
contact email	panjaa@utu.fi
lab head
Petra Miikkulainen
contact affiliation	University of Turku
contact email	petemi@utu.fi
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/07/PXD005453

PRIDE project URI

• PRIDE
  1. PXD005453
     1. Label: PRIDE project
     2. Name: Human ccRCC cell line 786-O with PHD3 knockdown LC-MS/MS