PXD005439

PXD005439 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomic profiling of Yersinia ruckeri strains
Description	Project goal is to identify proteomic profiles of Yersinia ruckeri, the causative agent of enteric redmouth disease in fish. Four strains (SP-05, CSF007-82, 7959-11 and YRNC-10) of Y. ruckeri were isolated from disease rainbow trout, Oncorhynchus mykiss. Strains, SP-05 and CSF007-82, belong to serotype 1 and biotype 1 (motile and lipase positive), while strains 7959-11 and YRNC-10 belong to serotype 1 and biotype 2 (non-motile and lipase negative) and belong to serotype 1. A single colony of each strain was inoculated into tryptic soy broth (Casein peptone, dipotassium hydrogen phosphate, glucose, sodium chloride, soya peptone, Sigma) and grown at 22 °C with shaking (150 rpm). These starter cultures were then diluted with fresh sterile tryptic soy broth to an optical density (OD 600) of 0.10 ± 0.05. Five hundred microlitres of the diluted starter cultures were inoculated in duplicates, into 25 ml of tryptic soy broth. Cultures were grown overnight at 22 °C with shaking (150 rpm) until the late log phase. Cells were harvested by centrifugation, then washed three times with PBS.
HostingRepository	PRIDE
AnnounceDate	2017-09-22
AnnouncementXML	Submission_2017-09-26_05:07:54.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gokhlesh Kumar
SpeciesList	scientific name: Yersinia ruckeri; NCBI TaxID: 29486;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-24 06:56:54	ID requested
1	2017-09-22 02:43:45	announced
⏵ 2	2017-09-26 05:07:55	announced	Updated publication reference for PubMed record(s): 28931430.

Kumar G, Hummel K, Welch TJ, Razzazi-Fazeli E, El-Matbouli M, Global proteomic profiling of Yersinia ruckeri strains. Vet Res, 48(1):55(2017) [pubmed]

curator keyword: Biological

submitter keyword: Yersinia ruckeri, enteric redmouth disease, Whole cell proteomics

Dr. Gokhlesh Kumar
contact affiliation	Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria
contact email	gokhlesh.kumar@vetmeduni.ac.at
lab head
Gokhlesh Kumar
contact affiliation	University of Veterinary Medicine, Vienna, Austria
contact email	Gokhlesh.Kumar@vetmeduni.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/09/PXD005439

PRIDE project URI

• PRIDE
  1. PXD005439
     1. Label: PRIDE project
     2. Name: Shotgun proteomic profiling of Yersinia ruckeri strains