PXD005438

PXD005438 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Complementary critical functions of Zfy1 and Zfy2 in mouse spermatogenesis and reproduction
Description	The mammalian Y chromosome plays a critical role in spermatogenesis. However, the exact functions of each gene in the Y chromosome have not been completely elucidated, partly owing to difficulties in gene targeting analysis of the Y chromosome. Zfy was first proposed to be a sex determination factor, but its function in spermatogenesis has been recently elucidated. Nevertheless, Zfy gene targeting analysis has not been performed thus far. Here, we adopted the highly efficient CRISPR/Cas9 system to generate individual Zfy1 or Zfy2 knockout (KO) mice and Zfy1 and Zfy2 double knockout (Zfy1/2-DKO) mice. While individual Zfy1 or Zfy2-KO mice did not show any significant phenotypic alterations in fertility, Zfy1/2-DKO mice were infertile and displayed abnormal sperm morphology, fertilization failure, and early embryonic development failure. Mass spectrometric screening, followed by confirmation with western blot analysis, showed that PLCZ1, PLCD4, PRSS21, and HTT protein expression were significantly deceased in spermatozoa of Zfy1/2-DKO mice compared with those of wild-type mice. These results are consistent with the phenotypic changes seen in the double-mutant mice. Collectively, our strategy and findings revealed that Zfy1 and Zfy2 have redundant functions in spermatogenesis, facilitating a better understanding of fertilization failure and early embryonic development failure.
HostingRepository	MassIVE
AnnounceDate	2017-01-11
AnnouncementXML	Submission_2017-01-11_17:40:53.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kumiko Shiozawa
SpeciesList	scientific name: Mus ; NCBI TaxID: 10088;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-24 02:25:45	ID requested
⏵ 1	2017-01-11 17:40:54	announced

Publication List

Takashi Nakasuji, Narumi Ogonuki, Tomoki Chiba, Tomomi Kato, Kumiko Shiozawa, Kenji Yamatoya, Hiromitsu Tanaka, Tadashi Kondo, Kenji Miyado, Naoyuki Miyasaka, Toshiro Kubota, Atsuo Ogura, and Hiroshi Asahara. Complementary critical functions of Zfy1 and Zfy2 in mouse spermatogenesis and reproduction. (in press).

Takashi Nakasuji, Narumi Ogonuki, Tomoki Chiba, Tomomi Kato, Kumiko Shiozawa, Kenji Yamatoya, Hiromitsu Tanaka, Tadashi Kondo, Kenji Miyado, Naoyuki Miyasaka, Toshiro Kubota, Atsuo Ogura, and Hiroshi Asahara. Complementary critical functions of Zfy1 and Zfy2 in mouse spermatogenesis and reproduction. (in press).

Keyword List

submitter keyword: Y chromosome, Zfy1, Zfy2, CRISPR/Cas9, spermatogenesis, LC-MSMS

submitter keyword: Y chromosome, Zfy1, Zfy2, CRISPR/Cas9, spermatogenesis, LC-MSMS

Contact List

principal_investigator
lab head
Kumiko Shiozawa
contact affiliation	National Cancer Centre Research Institute
contact email	kshiozaw@ncc.go.jp
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000080355