PXD005437

PXD005437 is an original dataset announced via ProteomeXchange.

Title	Comparative (phospho)proteomic profiling of wheat shoots from different cultivars
Description	Wheat is a cereal grain and one of the world’s major food crops. Recent advances in wheat genome sequencing are by now facilitating genomic and proteomic analyses of this crop. However, little is known about the protein levels of hexaploid versus tetraploid wheat cultivars, and knowledge on phosphorylated proteins still limited. Using our recently established (phospho)proteomic workflow, we performed a parallel analysis of the proteome and phosphoproteome on seedling leaves from two hexaploid wheat cultivars (Pavon 76 and USU-Apogee) and a tetraploid wheat (Senatore Cappelli). This revealed that a large portion of proteins and phosphosites can be quantified in all cultivars. Our shotgun proteomics data revealed a high similarity between hexaploid and tetraploid varieties with respect to protein abundance. However, we could identify a set of proteins that were differentially abundant between hexaploid and tetraploid cultivars. In addition, already at seedling stage, a small set of proteins were differential between the small (USU-Apogee) and larger hexaploid wheat cultivar (Pavon 76), which could potentially act as growth predictors. Finally, the phosphosites identified in this study can be retrieved from the in-house developed plant PTM-Viewer (bioinformatics.psb.ugent.be/webtools/ptm_viewer/), making this the first repository for phosphorylated wheat proteins. This paves the way for further in depth, quantitative (phospho)proteome-wide differential analyses upon a specific trigger or environmental change.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:34:10.676.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lam Dai Vu
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565; scientific name: Triticum turgidum subsp. durum; NCBI TaxID: 4567;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-24 01:45:16	ID requested
1	2017-04-03 02:26:24	announced
⏵ 2	2024-10-22 04:34:16	announced	2024-10-22: Updated project metadata.

10.3389/fpls.2017.00332;

Vu LD, Verstraeten I, Stes E, Van Bel M, Coppens F, Gevaert K, De Smet I, Proteome Profiling of Wheat Shoots from Different Cultivars. Front Plant Sci, 8():332(2017) [pubmed]

curator keyword: Biological

submitter keyword: proteomics, plant, phosphoproteomics,Wheat

Ive De Smet
contact affiliation	Department of Plant Systems Biology, VIB, 9052 Ghent, Belgium Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium
contact email	ivsme@psb.ugent.be
lab head
Lam Dai Vu
contact affiliation	VIB
contact email	lamvu@psb.ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD005437

PRIDE project URI

• PRIDE
  1. PXD005437
     1. Label: PRIDE project
     2. Name: Comparative (phospho)proteomic profiling of wheat shoots from different cultivars