PXD005436

PXD005436 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of human follicular fluid in poor ovarian responders during in vitro fertilization
Description	Poor ovarian response (POR) observed in controlled ovarian stimulation (COS) is often observed during in vitro fertilization and embryo transfer (IVF-ET) cycles and is a major problem. A POR has been found to be related to several factors, including advanced age, high body mass index, and history of ovarian or pelvic surgery. However, it is difficult to predict POR, as there are no specific biomarkers known. In this study, we used quantitative proteomic analyses to investigate potential biomarkers that can predict poor response during IVF based on follicular fluid samples. A total of 1,079 proteins were identified using a high-resolution orbitrap mass spectrometer coupled online to a nano flow liquid chromatography system. It is notable that 65 up-regulated and 66 down-regulated proteins were found to be functionally enriched in poor responders. We also validated these differentially expressed proteins (DEPs) using a triple-quadrupole (QQQ) mass spectrometer for quantification of targeted proteins. Of the DEPs, three proteins (pregnancy zone protein, renin, and sushi repeat-containing protein SRPX) were regarded as statistically significant (p < 0.05). These proteins (one known as an inhibitor of cell growth and another as a regulator of angiogenesis) are potential biomarkers candidates of POR in IVF.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:54.727.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jaewon Oh
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-24 01:42:40	ID requested
1	2018-07-04 15:22:56	announced
⏵ 2	2024-10-22 04:34:02	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201600333;

Oh JW, Kim SK, Cho KC, Kim MS, Suh CS, Lee JR, Kim KP, Proteomic analysis of human follicular fluid in poor ovarian responders during in vitro fertilization. Proteomics, 17(6):(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: Multiple reaction monitoring,Follicular fluid, Biomarker, Proteomics, Poor ovarian response

Kwang Pyo Kim
contact affiliation	Department of Applied Chemistry College of Applied Sciences, Kyung Hee University, Yongin-si, Gyeonggi-do, 446-701
contact email	kpkim@khu.ac.kr
lab head
Jaewon Oh
contact affiliation	Kyung Hee University
contact email	odinplate@naver.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD005436

PRIDE project URI

• PRIDE
  1. PXD005436
     1. Label: PRIDE project
     2. Name: Proteomic analysis of human follicular fluid in poor ovarian responders during in vitro fertilization