PXD005431

PXD005431 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Identification of ABL Kinase Substrates by Kinase Assay Linked Phosphoproteomics and Multiple Drug Treatments
Description	The kinase assay linked phosphoproteomics (KALIP) strategy was used to identify direct substrates of the Abelson tyrosine kinase (ABL). First, kinase assays using peptides and proteins from chronic myelogenous leukemia cells and recombinant ABL were done to identify kinase substrates in vitro. The ABL-dependent endogenous phosphoproteome was also examined using multiple drug treatments (imatinib, dasatinib, and bosutinib versus a vehicle treated control) and label-free quantitation after LC-MS/MS. The in vitro and in vivo substrate candidates were then compared to produce a list of highly confident direct ABL substrates.
HostingRepository	jPOST
AnnounceDate	2019-03-20
AnnouncementXML	Submission_2022-09-18_02:59:37.774.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Justine Arrington
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O4'-phospho-L-tyrosine; O-phospho-L-threonine; O-phospho-L-serine
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-23 15:33:52	ID requested
1	2019-03-20 15:32:45	announced
2	2021-02-04 17:02:28	announced	2021-02-04: Updated FTP location.
⏵ 3	2022-09-18 02:59:38	announced	2022-09-18: Updated FTP location.

Publication List

Arrington J, Xue L, Wang WH, Geahlen RL, Tao WA, Identification of the Direct Substrates of the ABL Kinase via Kinase Assay Linked Phosphoproteomics with Multiple Drug Treatments. J Proteome Res, 18(4):1679-1690(2019) [pubmed]

Arrington J, Xue L, Wang WH, Geahlen RL, Tao WA, Identification of the Direct Substrates of the ABL Kinase via Kinase Assay Linked Phosphoproteomics with Multiple Drug Treatments. J Proteome Res, 18(4):1679-1690(2019) [pubmed]

Keyword List

submitter keyword: Phosphorylation, kinase, cancer signaling

submitter keyword: Phosphorylation, kinase, cancer signaling

Contact List

W. Andy Tao
lab head
Justine Arrington
contact affiliation	Purdue University
dataset submitter

Full Dataset Link List

jPOST dataset URI
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000216/

jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000216/