PXD005428

PXD005428 is an original dataset announced via ProteomeXchange.

Title	Proteolytic processing of CD99
Description	The adhesion molecule CD99 is essential for transendothelial migration (TEM) of leukocytes. Here we demonstrate by biochemical and cellular assays that CD99 undergoes ectodomain shedding by the metalloprotease meprin β and subsequent intramembrane proteolysis by γ-secretase. The cleavage site in CD99 was identified by mass spectrometry within an acidic region highly conserved through different vertebrate species.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_05:42:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-23 07:51:20	ID requested
1	2017-01-02 12:19:19	announced
⏵ 2	2017-10-24 05:42:10	announced	Updated project metadata.

Bedau T, Peters F, Prox J, Arnold P, Schmidt F, Finkernagel M, K, ö, llmann S, Wichert R, Otte A, Ohler A, Stirnberg M, Lucius R, Koudelka T, Tholey A, Biasin V, Pietrzik CU, Kwapiszewska G, Becker-Pauly C, and promotes transendothelial cell migration. FASEB J, 31(3):1226-1237(2017) [pubmed]

curator keyword: Biological

submitter keyword: proteolysis, cell migration, inflammation, TEM, protease, adhesion molecule

Andreas Tholey
contact affiliation	Systematische Proteomics & Bioanalytik - Institut für Experimentelle Medizin Christian-Albrechts-Universität zu Kiel
contact email	A.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005428

PRIDE project URI

• PRIDE
  1. PXD005428
     1. Label: PRIDE project
     2. Name: Proteolytic processing of CD99