PXD005424

PXD005424 is an original dataset announced via ProteomeXchange.

Title	On tissue Top down microproteomic bridged to MALDI MS Imaging reveal the molecular physiome of brain regions
Description	Tissue Top-down microproteomic was performed on 3 brain regions. 156 references proteins from different cellular compartments were characterized. Moreover, 11 Alternative proteins issued from alternative open reading frames (AltORF) were identified and were relied to the brain regions and associated to the function of their reference proteins. Some proteins display specific post-translational modifications profiles or truncation linked the brain regions and their functions. Systemic biology performed on microproteome identified in each region allowed to associate sub-networks with functional physiology of each brain region. Back correlation of the local extracted and identified microproteome with tissue cellular localization was then performed by MALDI mass spectrometry imaging. 40 proteins including 4 Altproteins have been back correlated in MALDI MSI and are in line with their tissue extracted region and physiological function. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization.
HostingRepository	PRIDE
AnnounceDate	2017-11-14
AnnouncementXML	Submission_2017-11-14_08:25:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julien Franck
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-22 08:43:26	ID requested
⏵ 1	2017-11-14 08:25:09	announced

Delcourt V, Franck J, Quanico J, Gimeno JP, Wisztorski M, Raffo-Romero A, Kobeissy F, Roucou X, Salzet M, Fournier I, Spatially-Resolved Top-down Proteomics Bridged to MALDI MS Imaging Reveals the Molecular Physiome of Brain Regions. Mol Cell Proteomics, 17(2):357-372(2018) [pubmed]

curator keyword: Biological

submitter keyword: Top Down micro-proteomics, MALDI Imaging, Alternative Proteins

Michel Salzet
contact affiliation	Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM) - INSERM U1192 - Université Lille 1, Bât SN3, 1er étage, Cité Scientifique, F-59655 Villeneuve d’Ascq Cedex
contact email	michel.salzet@univ-lille1.fr
lab head
Julien Franck
contact affiliation	PRISM-INSERM U1192
contact email	julien.franck@univ-lille1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/11/PXD005424

PRIDE project URI

• PRIDE
  1. PXD005424
     1. Label: PRIDE project
     2. Name: On tissue Top down microproteomic bridged to MALDI MS Imaging reveal the molecular physiome of brain regions