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PXD005424

DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2017-11-14
  • AnnouncementXML: Submission_2017-11-14_08:25:08.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Julien Franck
  • Title: On tissue Top down microproteomic bridged to MALDI MS Imaging reveal the molecular physiome of brain regions
  • Description: Tissue Top-down microproteomic was performed on 3 brain regions. 156 references proteins from different cellular compartments were characterized. Moreover, 11 Alternative proteins issued from alternative open reading frames (AltORF) were identified and were relied to the brain regions and associated to the function of their reference proteins. Some proteins display specific post-translational modifications profiles or truncation linked the brain regions and their functions. Systemic biology performed on microproteome identified in each region allowed to associate sub-networks with functional physiology of each brain region. Back correlation of the local extracted and identified microproteome with tissue cellular localization was then performed by MALDI mass spectrometry imaging. 40 proteins including 4 Altproteins have been back correlated in MALDI MSI and are in line with their tissue extracted region and physiological function. Taken together, we established the molecular physiome of 3 rat brain regions through reference and hidden proteome characterization.
  • SpeciesList: scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
  • ModificationList: phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02016-11-22 08:43:26ID requested
12017-11-14 08:25:09announced

Publication List

  1. Delcourt V, Franck J, Quanico J, Gimeno JP, Wisztorski M, Raffo Romero A, Kobeissy F, Roucou X, Salzet M, Fournier I, Top-down microproteomics bridged to MALDI MS imaging reveals the molecular physiome of brain regions. Mol Cell Proteomics, ():(2017) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: Top Down micro-proteomics, MALDI Imaging, Alternative Proteins

Contact List

    Michel Salzet
    • contact affiliation: Laboratoire Protéomique, Réponse Inflammatoire et Spectrométrie de Masse (PRISM) - INSERM U1192 - Université Lille 1, Bât SN3, 1er étage, Cité Scientifique, F-59655 Villeneuve d’Ascq Cedex
    • contact email: michel.salzet@univ-lille1.fr
    • lab head:
    Julien Franck
    • contact affiliation: PRISM-INSERM U1192
    • contact email: julien.franck@univ-lille1.fr
    • dataset submitter:

Full Dataset Link List

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Repository Record List
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