PXD005419

PXD005419 is an original dataset announced via ProteomeXchange.

Title	Asian citrus psyllid gut LC-MSMS
Description	Huanglongbing, or citrus greening disease, has devastated the citrus industry. It is associated with the gram negative bacterium Candidatus Liberibacter asiaticus (CLas) that can be transmitted by Diaphorina citri, the Asian citrus psyllid. For transmission to occur, CLas must cross the gut of the ACP to circulate through the insect body. The insect gut is the first site of widespread interactions between the CLas and the ACP and forms a barrier to transmission. To investigate the effect of CLas exposure on this dynamic interface, we performed RNAseq and mass spectrometry-based proteomics to analyze the transcriptome and proteome respectively of dissected ACP guts. We found changes in iron metabolism, insecticide resistance, immune system, and apoptosis. We identified 83 long non-coding RNAs that are responsive to CLas, two of which have no homology to other organisms in NCBI. We also determined that Wolbachia, a symbiont of the ACP, undergoes protein regulation when CLas is present. Fluorescent in situ hybridization (FISH) confirmed that Wolbachia and CLas can inhabit the same ACP gut cell, but do not co-localize. These data provide a snapshot of the ACP gut under normal and CLas-exposed conditions, and provide tools to better understand the insect vector of the citrus greening pathosystem.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_12:47:47.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005419
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Angela Kruse
SpeciesList	scientific name: Diaphorina citri (Asian citrus psyllid); NCBI TaxID: 121845;
ModificationList	Methylthio; Oxidation
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-22 05:38:32	ID requested
⏵ 1	2018-10-26 12:47:48	announced

Kruse A, Fattah-Hosseini S, Saha S, Johnson R, Warwick E, Sturgeon K, Mueller L, MacCoss MJ, Shatters RG, Cilia Heck M, Combining 'omics and microscopy to visualize interactions between the Asian citrus psyllid vector and the Huanglongbing pathogen Candidatus Liberibacter asiaticus in the insect gut. PLoS One, 12(6):e0179531(2017) [pubmed]

curator keyword: Biological

submitter keyword: ACP, Diaphorina citri, gut, citrus, psyllid, LC-MSMS, Wolbachia, Candidatus Liberibacter asiaticus

Michelle Cilia
contact affiliation	USDA-ARS, Boyce Thompson Institute, Cornell University
contact email	mlc68@cornell.edu
lab head
Angela Kruse
contact affiliation	Cornell University
contact email	ark253@cornell.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005419

PRIDE project URI

• PRIDE
  1. PXD005419
     1. Label: PRIDE project
     2. Name: Asian citrus psyllid gut LC-MSMS