PXD005415

PXD005415 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic analysis indicates that the B cell cytoskeleton is disrupted after exposure to mercury
Description	Exposure to environmental mercury (Hg) currently may contribute to immune system dysfunction and autoimmune disease. In this study phosphoproteomic analysis was used to investigate the impact of Hg2+ on a B cell line. Treatments were with 0, 2, 5, 10, 20, 50 and 100 uM Hg2+. 1713 phosphoproteins and 1937 confidently localized phosphorylation sites were identified. 161 phosphoproteins responded to Hg2+ treatment (ANOVA, 10% FDR). Hg2+ at 50 and 100 uM stimulated Tyr phosphorylation on residues in the B-cell receptor complex as well as other systems. At 20 uM and below Hg2+ primarily caused hyperphosphorylation of pSer/Thr residues and affected cytoskeletal systems. Map Kinase 1 was exceptional as it was hyperphosphorylated at Tyr 185 following 20 uM and lower Hg2+ treatments. These results contribute novel insight into the mechanism for immune system disruption by Hg2+.
HostingRepository	PRIDE
AnnounceDate	2020-06-12
AnnouncementXML	Submission_2020-06-11_16:23:53.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005415
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Nicholas Carruthers
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	Ammonia-loss; Phospho; Deamidated; Dehydrated; Oxidation; Acetyl; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-21 06:47:21	ID requested
⏵ 1	2020-06-11 16:23:54	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: mercury, phosphoproteomics, phosphoproteome, Q Exactive

Paul M Stemmer
contact affiliation	Institute of Environmental Health Sciences Wayne State University
contact email	pmstemmer@wayne.edu
lab head
Nicholas Carruthers
contact affiliation	IEHS
contact email	aj7682@wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005415
     1. Label: PRIDE project
     2. Name: Phosphoproteomic analysis indicates that the B cell cytoskeleton is disrupted after exposure to mercury