PXD005407

PXD005407 is an original dataset announced via ProteomeXchange.

Title	Tumor suppressor TAp73 is required to maintain pluripotency of cancer stem cells
Description	Cancer stem cells (CSCs) are resistant to conventional chemotherapy and are hence responsible for cancer relapse. Pluripotency is a characteristic of CSCs which allows them to rapidly proliferate while maintaining the ability to differentiate into various lineages. We found that TAp73, but not its homologue p53, is required for the pluripotency of CSCs. TAp73 knockdown (KD) decreased SOD1 levels, increased ROS production and disturbed metabolism which induced differentiation and abrogated pluripotency in CSCs. TAp73 related decrease in pluripotency is linked to increased autophagy and senescence in CSCs. Furthermore, TAp73 KD also decreased the levels of pluripotency factor Sox-2 within heterogeneous cancer cell lines. Interestingly, TAp73-deficient CSCs strongly lose tumorigenic potential in mice and tumors that did form had significantly lower levels of SOD1 and pluripotency marker Oct4. Our findings reveal a unique role of TAp73 in CSCs development that is important to consider while devising future therapeutic strategies against cancer.
HostingRepository	PRIDE
AnnounceDate	2019-03-05
AnnouncementXML	Submission_2019-03-05_04:07:11.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005407
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Patrick Murphy
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex; Oxidation; Carbamidomethyl
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-21 04:28:38	ID requested
⏵ 1	2019-03-05 04:07:12	announced

Sharif T, Dai C, Martell E, Ghassemi-Rad MS, Hanes MR, Murphy PJ, Kennedy BE, Venugopal C, Subapanditha M, Giacomantonio CA, Marcato P, Singh SK, Gujar S, TAp73 Modifies Metabolism and Positively Regulates Growth of Cancer Stem-Like Cells in a Redox-Sensitive Manner. Clin Cancer Res, 25(6):2001-2017(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Stem Cells, p73

Dr. Shashi Gujar
contact affiliation	Departments of Pathology, Microbiology and Immunology, Dalhousie University
contact email	shashi.gujar@dal.ca
lab head
Patrick Murphy
contact affiliation	Mass Spectrometry
contact email	pitter9@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005407
     1. Label: PRIDE project
     2. Name: Tumor suppressor TAp73 is required to maintain pluripotency of cancer stem cells