PXD005404

PXD005404 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic analysis of hemolymph from uninfected and Candidatus Liberibacter asiaticus-infected Diaphorina citri
Description	Hemolymph was characterized from Diaphorina citri adults infected with the phytopathogen, Candidatus Liberibacter asiaticus (CLas) and compared with that from uninfected psyllids. This study identified 5531 and 3220 peptides within infected and uninfected hemolymph using nano LC-MS/MS. A reduced number of proteins were detected for D. citri and all known endosymbionts within infected hemolymph as compared to uninfected hemolymph. A large number of immune defense proteins were absent from D. citri hemolymph; however, a single recognition protein (PGRP), two serine protease inhibitors, three prophenoloxidase (proPO) enzymes, and a single serine protease in an uninfected D. citri were detected. The hemolymph is nearly devoid of nutrient storage proteins. This is the first proteomic analysis of D. citri hemolymph that also analyzes the components contributed by all of the endosymbionts. By comparing the contribution of each endosymbiont (CCR, CPA,WB) in the presence and absence of CLas infection, this study provides initial insights regarding the hemolymph response to microbial community shifts associated with D. citri infection status. Our data also present potential protein targets for analysis and disruption of CLas transmission that may facilitate management of huanglongbing (HLB) caused by CLas in citrus.
HostingRepository	PRIDE
AnnounceDate	2017-01-18
AnnouncementXML	Submission_2017-01-18_04:05:27.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005404
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Torrence Gill
SpeciesList	scientific name: Candidatus Profftella armatura; NCBI TaxID: 669502; scientific name: Liberibacter endosymbiont of Diaphorina citri; NCBI TaxID: 116597; scientific name: Candidatus Carsonella ruddii DC; NCBI TaxID: 667013; scientific name: Wolbachia endosymbiont of Diaphorina citri; NCBI TaxID: 116598; scientific name: Diaphorina citri (Asian citrus psyllid); NCBI TaxID: 121845;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-21 04:19:44	ID requested
⏵ 1	2017-01-18 04:05:29	announced

Gill TA, Chu C, Pelz-Stelinski KS, Comparative proteomic analysis of hemolymph from uninfected and Candidatus Liberibacter asiaticus-infected Diaphorina citri. Amino Acids, 49(2):389-406(2017) [pubmed]

curator keyword: Biological

submitter keyword: Asian Citrus Psyllid, Diaphorina citri, Endosymbionts, Hemolymph, Hemiptera

Kirsten Pelz-Stelinski
contact affiliation	University of Florida Department of Entomology Citrus Research and Education Center Lake Alfred, Florida
contact email	pelzstelinski@ufl.edu
lab head
Torrence Gill
contact affiliation	University of Florida
contact email	gilltorrence@hotmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005404
     1. Label: PRIDE project
     2. Name: Comparative proteomic analysis of hemolymph from uninfected and Candidatus Liberibacter asiaticus-infected Diaphorina citri