PXD005400

PXD005400 is an original dataset announced via ProteomeXchange.

Title	Composition and immune environment of urinary bladder matrix scaffolds
Description	Urinary bladder matrix (UBM) is used clinically for treatment of diabetic ulcers, abdominal wall reconstruction, soft tissue filling among other applications. Composed mainly of collagen, the material is a mix of fibrous collagen structures and sheet-forming structures characteristic of the urinary bladder matrix basal lamina. Upon application of a biomaterial in a traumatic or non-traumatic setting, there is a cascade of immune cells that react to the damaged tissue and biomaterial. Here, through the use of multicolor flow cytometry, we describe the various cells that infiltrate the UBM scaffold in subcutaneous and volumetric muscle injury model. A wide variety of immune cells are found in the UBM scaffold immune microenvironment (SIM) including F4/80 + macrophages, CD11c + dendritic cells, CD3 + T cells and CD19 + B cells. UBM induced a systemic IL-4 upregulation and a local M2-macrophage response. The recruitment and activation of these cells is dependent upon signals from the scaffold and communication between the different cell types present.
HostingRepository	PRIDE
AnnounceDate	2017-06-09
AnnouncementXML	Submission_2017-06-09_00:00:58.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-21 01:50:42	ID requested
⏵ 1	2017-06-09 00:00:59	announced

Sadtler K, Sommerfeld SD, Wolf MT, Wang X, Majumdar S, Chung L, Kelkar DS, Pandey A, Elisseeff JH, Proteomic composition and immunomodulatory properties of urinary bladder matrix scaffolds in homeostasis and injury. Semin Immunol, 29():14-23(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: ECM, urinary bladder, collagen

Jennifer H. Elisseeff
contact affiliation	Translational Tissue Engineering Center, Wilmer Eye Institute and Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21287, USA. ( lab head )
contact email	jhe@jhu.edu
lab head
Akhilesh Pandey
contact affiliation	Johns Hopkins University
contact email	pandey@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/06/PXD005400

PRIDE project URI

• PRIDE
  1. PXD005400
     1. Label: PRIDE project
     2. Name: Composition and immune environment of urinary bladder matrix scaffolds