PXD005364

PXD005364 is an original dataset announced via ProteomeXchange.

Title	Improved MS-based proteomics by the reduction of isotopic composition in vivo
Description	Reducing the isotopic composition of proteins in vivo improves in-depth, high resolution MS-based quantitative proteomics. We used U-[12C]-glucose as the metabolic precursor of all amino acids in yeast. This substantially increased the peptide monoisotopic ion intensity in bottom-up analyses, greatly improving identification scores and protein sequence coverage, and making it possible to address the dynamics of protein turnover at the proteome scale.
HostingRepository	PRIDE
AnnounceDate	2017-08-24
AnnouncementXML	Submission_2017-08-24_04:11:06.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Léger
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-16 06:05:13	ID requested
⏵ 1	2017-08-24 04:11:08	announced

L, é, ger T, Garcia C, Collomb L, Camadro JM, . Mol Cell Proteomics, 16(11):2017-2031(2017) [pubmed]

curator keyword: Technical

submitter keyword: Bottom-up MS-based proteomics, Candida albicans, [U-12C]-carbon source, protein half-life

Jean-Michel Camadro
contact affiliation	"Mitochondria, Metals and Oxidative Stress" group leader Head "Mass Spectrometry Lab and Proteomics core Facility" Jacques Monod Institute UMR7592 CNRS - Paris Diderot University 15 rue Hélène Brion 75205 Paris Cedex 13, FRANCE tel : 33(1)57278029
contact email	jean-michel.camadro@ijm.fr
lab head
Thibaut Léger
contact affiliation	Institut Jacques Monod
contact email	leger@ijm.univ-paris-diderot.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/08/PXD005364

PRIDE project URI

• PRIDE
  1. PXD005364
     1. Label: PRIDE project
     2. Name: Improved MS-based proteomics by the reduction of isotopic composition in vivo