PXD005362

PXD005362 is an original dataset announced via ProteomeXchange.

Title	Increased Hemodynamic Load in Early Embryonic Stages Alters Endocardial to Mesenchymal Transition
Description	Normal blood flow is essential for proper heart formation during embryonic development, as abnormal hemodynamic load (blood pressure and shear stress) results in cardiac defects seen in congenital heart disease. However, the progressive detrimental remodeling processes that relate altered blood flow to cardiac defects remain unclear. Outflow tract banding was used to increase hemodynamic load in the chicken embryo heart between Hamburger and Hamilton stages 18 and 24. Increased hemodynamic load induced increased cell density in outflow tract cushions, fewer cells along the endocardial lining, endocardium junction disruption, and altered periostin expression as measured by confocal microscopy analysis. Proteomic mass-spectrometry analysis quantified altered protein composition after banding.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_12:55:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sandra Rugonyi
SpeciesList	scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-16 03:46:20	ID requested
⏵ 1	2018-10-26 12:55:13	announced

Midgett M, L, ó, pez CS, David L, Maloyan A, Rugonyi S, Increased Hemodynamic Load in Early Embryonic Stages Alters Myofibril and Mitochondrial Organization in the Myocardium. Front Physiol, 8():631(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: heart development

chick embryos

hemodynamics

increased load

10-plex TMT labeling

shotgun proteomics

Sandra Rugonyi
contact affiliation	Biomedical Engineering Department Oregon Health & Science University Portland, OR 97239
contact email	rugonyis@ohsu.edu
lab head
Sandra Rugonyi
contact affiliation	Oregon Health & Science University
contact email	rugonyis@ohsu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005362

PRIDE project URI

• PRIDE
  1. PXD005362
     1. Label: PRIDE project
     2. Name: Increased Hemodynamic Load in Early Embryonic Stages Alters Endocardial to Mesenchymal Transition