PXD005356

PXD005356 is an original dataset announced via ProteomeXchange.

Title	Dbf4-dependent kinase (DDK) and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis
Description	DNA repair by homologous recombination is under stringent cell cycle control. This includes the last step of the reaction, disentanglement of DNA joint molecules (JMs). Previous work has established that JMs resolving nucleases are activated specifically at the onset of mitosis. In case of budding yeast Mus81-Mms4, this cell cycle stage-specific activation is known to depend on phosphorylation by CDK and Cdc5 kinases. Here, we show that a third cell cycle kinase, Cdc7-Dbf4 (DDK), targets Mus81-Mms4 in conjunction with Cdc5 - both kinases bind to as well as phosphorylate Mus81-Mms4 in an interdependent manner. Moreover, DDK-mediated phosphorylation of Mms4 is strictly required for Mus81 activation in mitosis, establishing DDK as a novel regulator of homologous recombination. The scaffold protein Rtt107, which is part of the Mus81-Mms4 complex, interacts with Cdc7 and thereby targets DDK and Cdc5 to the complex enabling full Mus81 activation. Therefore, Mus81 activation in mitosis involves at least three cell cycle kinases, Cdk1, Cdc5 and DDK. Furthermore, tethering of the kinases in a stable complex with Mus81 is critical for efficient JM resolution
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:34:34.010.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nagarjuna Nagaraj
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-15 05:22:40	ID requested
1	2017-01-23 01:28:15	announced
⏵ 2	2024-10-22 04:34:34	announced	2024-10-22: Updated project metadata.

10.15252/embj.201694831;

Princz LN, Wild P, Bittmann J, Aguado FJ, Blanco MG, Matos J, Pfander B, Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis. EMBO J, 36(5):664-678(2017) [pubmed]

curator keyword: Biological

submitter keyword: DDK, yeast,DNA repair

Boris Pfander
contact affiliation	DNA Replication & Genome Integrity Group Max-Planck Institute of Biochemistry Am Klopferspitz 18 82152 Martinsried, Germany ( lab head )
contact email	bpfander@biochem.mpg.de
lab head
Nagarjuna Nagaraj
contact affiliation	MaxPlanck Institute for Biochemistry, Core facility
contact email	nagaraj@biochem.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005356
     1. Label: PRIDE project
     2. Name: Dbf4-dependent kinase (DDK) and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis