PXD005349

PXD005349 is an original dataset announced via ProteomeXchange.

Title	Combining Affinity Enrichment, Cross-Linking with Photo-Amino Acids, and Mass Spectrometry for Probing Protein Kinase D2 Interactions
Description	We present an approach that relies on the affinity enrichment of a target protein from a complex mixture, followed by a UV-induced activation of incorporated diazirine photo-reactive amino acids (photo-methionine and photo-leucine) and a covalent fixation of interaction partners. The captured protein complexes are enzymatically digested and interacting proteins are identified and quantified by label-free LC/MS analysis. Using HeLa cell lysates with photo-methionine and photo-leucine-labeled proteins, we were able to capture and preserve protein interactions that are otherwise elusive in conventional pull-down experiments. Our approach is exemplified for mapping the protein interaction network of protein kinase D2, but can be applied to any protein system.
HostingRepository	PRIDE
AnnounceDate	2017-04-10
AnnouncementXML	Submission_2017-04-10_01:27:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Björn Häupl
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-15 02:26:36	ID requested
⏵ 1	2017-04-10 01:27:20	announced

H, ä, upl B, Ihling CH, Sinz A, Combining affinity enrichment, cross-linking with photo amino acids, and mass spectrometry for probing protein kinase D2 interactions. Proteomics, 17(10):e1600459(2017) [pubmed]

curator keyword: Technical

submitter keyword: affinity enrichment, photo-cross-linking, photo-methionine, photo-leucine, protein interaction, PKD2, Orbitrap Fusion

Andrea Sinz
contact affiliation	Department of Pharmaceutical Chemistry and BioanalyticsInstitute of Pharmacy Martin Luther University Halle-Wittenberg Wolfgang-Langenbeck-St. 4 D-06120 Halle (Saale), Germany
contact email	andrea.sinz@pharmazie.uni-halle.de
lab head
Björn Häupl
contact affiliation	Martin Luther University Halle-Wittenberg
contact email	bjoern.haeupl@pharmazie.uni-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/04/PXD005349

PRIDE project URI

• PRIDE
  1. PXD005349
     1. Label: PRIDE project
     2. Name: Combining Affinity Enrichment, Cross-Linking with Photo-Amino Acids, and Mass Spectrometry for Probing Protein Kinase D2 Interactions