PXD005340

PXD005340 is an original dataset announced via ProteomeXchange.

Title	Targeted analysis of IGF:s in cat serum, LC-MS/MS
Description	Today immunoassays are widely used in veterinary medicine, but lack of species specific assays often necessitates the use of assays developed for human applications. Mass spectrometry (MS) is an attractive alternative due to high specificity and versatility, allowing for species-independent analysis. Targeted MS-based quantification methods are valuable complements to large scale shotgun analysis. A method referred to as parallel reaction monitoring (PRM), implemented on Orbitrap MS, has lately been presented as an excellent alternative to more traditional selected reaction monitoring/multiple reaction monitoring (SRM/MRM)methods. The insulin-like growth factor (IGF)-system is not well described in the cat but there are indications of important differences between cats and humans. In feline medicine IGF–I is mainly analyzed for diagnosis of growth hormone disorders but also for research, while the other proteins in the IGF-system are not routinely analyzed within clinical practice. Here, a PRM method for quantification of IGF–I, IGF–II, IGF binding protein (BP) –3 and IGFBP–5 in feline serum is presented. Selective quantification was supported by the use of a newly launched internal standard named QPrEST™. Homology searches demonstrated the possibility to use this standard of human origin for quantification of the targeted feline proteins. Excellent quantitative sensitivity at the attomol/µL (pM) level and selectivity were obtained. As the presented approach is very generic we show that high resolution mass spectrometry in combination with PRM and QPrEST™ internal standards is a versatile tool for protein quantitation across multispecies.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:31:41.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Margareta Ramstrom
SpeciesList	scientific name: Felis catus (Cat) (Felis silvestris catus); NCBI TaxID: 9685;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-14 03:41:12	ID requested
1	2017-01-04 06:25:49	announced
⏵ 2	2017-10-24 04:31:43	announced	Updated project metadata.

Sundberg M, Strage EM, Bergquist J, Holst BS, Ramstr, ö, m M, Quantitative and Selective Analysis of Feline Growth Related Proteins Using Parallel Reaction Monitoring High Resolution Mass Spectrometry. PLoS One, 11(12):e0167138(2016) [pubmed]

curator keyword: Biomedical

submitter keyword: Cat, LC-MS/MS, PRM

Margareta Ramstrom
contact affiliation	Analytical Chemistry, Department of Chemistry – BMC and Science for Life Laboratory, Uppsala University, Box 599, 751 24 Uppsala, Sweden
contact email	margareta.ramstrom@kemi.uu.se
lab head
Margareta Ramstrom
contact affiliation	Uppsala University
contact email	margareta.ramstrom@kemi.uu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005340

PRIDE project URI

• PRIDE
  1. PXD005340
     1. Label: PRIDE project
     2. Name: Targeted analysis of IGF:s in cat serum, LC-MS/MS