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PXD005324

PXD005324 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleVariations of Metabolites and Proteome from Lonicera japonica Thunb. Buds Blossom to Flowers under UV Radiation
DescriptionLonicera japonica Thunb., known as Jin Yin Hua or Japanese honeysuckle, is an herbal medicine in Asian countries. Its flowers have been used as folk medicine for clinical practice or used as food or making healthy beverage for 1500 years in China. To investigate the molecular developmental processes from L. japonica buds to flowers under UV radiation, comparative proteomics analyses of buds and flowers were performed. Fifty-four differential proteins were identified including 42 increased proteins and 12 decreased proteins. The abundance of proteins related to glycolysis, TCA/organic acid transformation, major carbohydrate metabolism, oxidative pentose phosphate, stress, secondary metabolism, hormone, and mitochondrial electron transport were increased during flower opening process under UV radiation. Six metabolites were identified and relatively quantified by LC-MS/MS in L. japonica buds and flowers. The 1,1-diphenyl-2-picrylhydrazyl assay revealed that antioxidant activity of L. japonica buds was better than that of flowers. These results suggest that UV-B radiation could induce the production of endogenous ethylene in L. japonica buds, which facilitate the buds blossom and activate the antioxidant system. Additionally, the higher content of metabolites and antioxidant capability in L. japonica buds indicates that L. japonica buds stage might be the better harvest time compared to the flower.
HostingRepositoryPRIDE
AnnounceDate2017-01-18
AnnouncementXMLSubmission_2017-01-18_01:48:53.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWei Zhu
SpeciesList scientific name: Lonicera japonica; NCBI TaxID: 105884;
ModificationListiodoacetamide derivatized residue
Instrument4800 Plus MALDI TOF/TOF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-11-11 03:44:47ID requested
12017-01-18 01:48:54announced
Publication List
Zhu W, Zheng W, Hu X, Xu X, Zhang L, Tian J, Variations of metabolites and proteome in Lonicera japonica Thunb. buds and flowers under UV radiation. Biochim Biophys Acta Proteins Proteom, 1865(4):404-413(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Lonicera japonica Thunb., proteomics, LC-MS/MS, flower processing, UV radiation
Contact List
Wei Zhu
contact affiliationCollege of Biomedical Engineering & Instrument Science, Zhejiang University
contact emailrutin@zju.edu.cn
lab head
Wei Zhu
contact affiliationZhejiang University
contact emailrutin@zju.edu.cn
dataset submitter
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