PXD005324 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Variations of Metabolites and Proteome from Lonicera japonica Thunb. Buds Blossom to Flowers under UV Radiation |
Description | Lonicera japonica Thunb., known as Jin Yin Hua or Japanese honeysuckle, is an herbal medicine in Asian countries. Its flowers have been used as folk medicine for clinical practice or used as food or making healthy beverage for 1500 years in China. To investigate the molecular developmental processes from L. japonica buds to flowers under UV radiation, comparative proteomics analyses of buds and flowers were performed. Fifty-four differential proteins were identified including 42 increased proteins and 12 decreased proteins. The abundance of proteins related to glycolysis, TCA/organic acid transformation, major carbohydrate metabolism, oxidative pentose phosphate, stress, secondary metabolism, hormone, and mitochondrial electron transport were increased during flower opening process under UV radiation. Six metabolites were identified and relatively quantified by LC-MS/MS in L. japonica buds and flowers. The 1,1-diphenyl-2-picrylhydrazyl assay revealed that antioxidant activity of L. japonica buds was better than that of flowers. These results suggest that UV-B radiation could induce the production of endogenous ethylene in L. japonica buds, which facilitate the buds blossom and activate the antioxidant system. Additionally, the higher content of metabolites and antioxidant capability in L. japonica buds indicates that L. japonica buds stage might be the better harvest time compared to the flower. |
HostingRepository | PRIDE |
AnnounceDate | 2017-01-18 |
AnnouncementXML | Submission_2017-01-18_01:48:53.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Wei Zhu |
SpeciesList | scientific name: Lonicera japonica; NCBI TaxID: 105884; |
ModificationList | iodoacetamide derivatized residue |
Instrument | 4800 Plus MALDI TOF/TOF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-11-11 03:44:47 | ID requested | |
⏵ 1 | 2017-01-18 01:48:54 | announced | |
Publication List
Zhu W, Zheng W, Hu X, Xu X, Zhang L, Tian J, Variations of metabolites and proteome in Lonicera japonica Thunb. buds and flowers under UV radiation. Biochim Biophys Acta Proteins Proteom, 1865(4):404-413(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Lonicera japonica Thunb., proteomics, LC-MS/MS, flower processing, UV radiation |
Contact List
Wei Zhu |
contact affiliation | College of Biomedical Engineering & Instrument Science, Zhejiang University |
contact email | rutin@zju.edu.cn |
lab head | |
Wei Zhu |
contact affiliation | Zhejiang University |
contact email | rutin@zju.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005324
- Label: PRIDE project
- Name: Variations of Metabolites and Proteome from Lonicera japonica Thunb. Buds Blossom to Flowers under UV Radiation