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PXD005312

PXD005312 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeep Phosphoproteomic measurements pinpointing drug induced protective mechanisms in neuro-2a cells
DescriptionAlzheimer's disease (AD) is a progressive and irreversible neurological disorder which impairs the living quality of old people and even life spans. New compounds have shown potential inneuroprotective effects in AD, such as GFKP-19, which is a 2-pyrrolidone derivative, which has been proved to enhance the memory of dysmnesia mouse. The molecular mechanisms remains to be established for these drug candidates. Large-scale phosphoproteomic approach has been evolved rapidly in the last several years, which should provide useful toolkit to understand cellular signaling underlying drug effects. To establish and test such a method framework, we accurately analyzed the deep quantitative phosphoproteome of the neuro-2a cells treated with or without GFKP-19 using triple SILAC labeling. A total of 14,761 class I phosphosites were quantified between control, damaged, and protected conditions using the high resolution mass spectrometry, with a high inter-mass spectrometer reproducibility for even subtle regulation events. Our data suggests that GFKP-19 can reverse Aβ25-35 caused phosphorylation change in neuro-2a cells, and might perform the neuronal protective effect by decreasing tau protein phosphorylation through down-regulating the phosphorylation level of MAPK14 at T180.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:33:12.379.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChengli Yu
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-11-10 06:49:57ID requested
12017-01-16 13:03:26announced
22024-10-22 04:33:13announced2024-10-22: Updated project metadata.
Publication List
Yu C, Gao J, Zhou Y, Chen X, Xiao R, Zheng J, Liu Y, Zhou H, Deep Phosphoproteomic Measurements Pinpointing Drug Induced Protective Mechanisms in Neuronal Cells. Front Physiol, 7():635(2016) [pubmed]
10.3389/fphys.2016.00635;
Keyword List
curator keyword: Biomedical
submitter keyword: Neuro-2a, LC-MSMS, phosphoproteome
Contact List
Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research
contact affiliationShanghai Institute of Materia Medica, Chinese Academy of Sciences
contact emailzhouhu@simm.ac.cn
lab head
Chengli Yu
contact affiliationShanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
contact emailyuchengli@simm.ac.cn
dataset submitter
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Dataset FTP location
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