PXD005312

PXD005312 is an original dataset announced via ProteomeXchange.

Title	Deep Phosphoproteomic measurements pinpointing drug induced protective mechanisms in neuro-2a cells
Description	Alzheimer's disease (AD) is a progressive and irreversible neurological disorder which impairs the living quality of old people and even life spans. New compounds have shown potential inneuroprotective effects in AD, such as GFKP-19, which is a 2-pyrrolidone derivative, which has been proved to enhance the memory of dysmnesia mouse. The molecular mechanisms remains to be established for these drug candidates. Large-scale phosphoproteomic approach has been evolved rapidly in the last several years, which should provide useful toolkit to understand cellular signaling underlying drug effects. To establish and test such a method framework, we accurately analyzed the deep quantitative phosphoproteome of the neuro-2a cells treated with or without GFKP-19 using triple SILAC labeling. A total of 14,761 class I phosphosites were quantified between control, damaged, and protected conditions using the high resolution mass spectrometry, with a high inter-mass spectrometer reproducibility for even subtle regulation events. Our data suggests that GFKP-19 can reverse Aβ25-35 caused phosphorylation change in neuro-2a cells, and might perform the neuronal protective effect by decreasing tau protein phosphorylation through down-regulating the phosphorylation level of MAPK14 at T180.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:12.379.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chengli Yu
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-10 06:49:57	ID requested
1	2017-01-16 13:03:26	announced
⏵ 2	2024-10-22 04:33:13	announced	2024-10-22: Updated project metadata.

Yu C, Gao J, Zhou Y, Chen X, Xiao R, Zheng J, Liu Y, Zhou H, Deep Phosphoproteomic Measurements Pinpointing Drug Induced Protective Mechanisms in Neuronal Cells. Front Physiol, 7():635(2016) [pubmed]

10.3389/fphys.2016.00635;

curator keyword: Biomedical

submitter keyword: Neuro-2a, LC-MSMS, phosphoproteome

Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences
contact email	zhouhu@simm.ac.cn
lab head
Chengli Yu
contact affiliation	Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China
contact email	yuchengli@simm.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005312

PRIDE project URI

• PRIDE
  1. PXD005312
     1. Label: PRIDE project
     2. Name: Deep Phosphoproteomic measurements pinpointing drug induced protective mechanisms in neuro-2a cells