PXD005312 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Deep Phosphoproteomic measurements pinpointing drug induced protective mechanisms in neuro-2a cells |
Description | Alzheimer's disease (AD) is a progressive and irreversible neurological disorder which impairs the living quality of old people and even life spans. New compounds have shown potential inneuroprotective effects in AD, such as GFKP-19, which is a 2-pyrrolidone derivative, which has been proved to enhance the memory of dysmnesia mouse. The molecular mechanisms remains to be established for these drug candidates. Large-scale phosphoproteomic approach has been evolved rapidly in the last several years, which should provide useful toolkit to understand cellular signaling underlying drug effects. To establish and test such a method framework, we accurately analyzed the deep quantitative phosphoproteome of the neuro-2a cells treated with or without GFKP-19 using triple SILAC labeling. A total of 14,761 class I phosphosites were quantified between control, damaged, and protected conditions using the high resolution mass spectrometry, with a high inter-mass spectrometer reproducibility for even subtle regulation events. Our data suggests that GFKP-19 can reverse Aβ25-35 caused phosphorylation change in neuro-2a cells, and might perform the neuronal protective effect by decreasing tau protein phosphorylation through down-regulating the phosphorylation level of MAPK14 at T180. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:33:12.379.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Chengli Yu |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-11-10 06:49:57 | ID requested | |
1 | 2017-01-16 13:03:26 | announced | |
⏵ 2 | 2024-10-22 04:33:13 | announced | 2024-10-22: Updated project metadata. |
Publication List
Yu C, Gao J, Zhou Y, Chen X, Xiao R, Zheng J, Liu Y, Zhou H, Deep Phosphoproteomic Measurements Pinpointing Drug Induced Protective Mechanisms in Neuronal Cells. Front Physiol, 7():635(2016) [pubmed] |
10.3389/fphys.2016.00635; |
Keyword List
curator keyword: Biomedical |
submitter keyword: Neuro-2a, LC-MSMS, phosphoproteome |
Contact List
Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research |
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences |
contact email | zhouhu@simm.ac.cn |
lab head | |
Chengli Yu |
contact affiliation | Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China |
contact email | yuchengli@simm.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/01/PXD005312 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005312
- Label: PRIDE project
- Name: Deep Phosphoproteomic measurements pinpointing drug induced protective mechanisms in neuro-2a cells