PXD005311

PXD005311 is an original dataset announced via ProteomeXchange.

Title	Large scale phosphoprotein profiling to unravel Drosophila cold acclimation regulatory mechanisms
Description	In this study we compared the phosphoproteome of control and cold acclimated Drosophila melanogaster in order to unravel the cold acclimation regulation mechanisms. For this prurpose we enriched phosphopeptides from each sample using a sequential elution from IMAC strategy (SIMAC) and analyzed them with a LTQ-OrbitrapXL mass spectrometer using a combination of different fragmentation strategies (classical data dependant analysis using CID, neutral loss scanning and multi-stage activation) in order to improve the number of identified phosphopeptides.
HostingRepository	PRIDE
AnnounceDate	2017-05-16
AnnouncementXML	Submission_2017-05-16_09:10:13.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005311
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	E Com
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	phosphorylated residue; monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-10 06:41:24	ID requested
⏵ 1	2017-05-16 09:10:15	announced

Colinet H, Pineau C, Com E, Large scale phosphoprotein profiling to explore Drosophila cold acclimation regulatory mechanisms. Sci Rep, 7(1):1713(2017) [pubmed]

curator keyword: Biological

submitter keyword: Phosphorylation network, acclimation, SIMAC, fruit fly

Charles Pineau
contact affiliation	Protim - Irset – Inserm U1085 Campus de Beaulieu, Rennes, France
contact email	charles.pineau@inserm.fr
lab head
E Com
contact affiliation	Plate-Forme protéomique Biogenouest
contact email	emmanuelle.com@univ-rennes1.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/05/PXD005311

PRIDE project URI

• PRIDE
  1. PXD005311
     1. Label: PRIDE project
     2. Name: Large scale phosphoprotein profiling to unravel Drosophila cold acclimation regulatory mechanisms