PXD005309

PXD005309 is an original dataset announced via ProteomeXchange.

Title	SPOP mutation
Description	Recurrent point mutations in SPOP define a distinct molecular subclass of prostate cancer. Here, we describe the first mouse model showing that mutant SPOP drives prostate tumorigenesis in vivo. Conditional expression of mutant SPOP in the prostate dramatically altered phenotypes in the setting of Pten loss, with early neoplastic lesions (high-grade prostatic intraepithelial neoplasia) with striking nuclear atypia, and invasive poorly differentiated carcinoma. In mouse prostate organoids, mutant SPOP drove increased proliferation and a transcriptional signature consistent with human prostate cancer. Using these models and human prostate cancer samples, we show that SPOP mutation activates both PI3K/mTOR and androgen receptor (AR) signaling, effectively uncoupling the normal negative feedback between these two pathways. Associated RNA-seq data deposited in GEO: GSE94839.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_04:05:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anton Poliakov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-10 03:40:52	ID requested
1	2017-03-20 09:40:47	announced
2	2017-03-22 01:30:45	announced	Updated project metadata.
⏵ 3	2017-10-24 04:05:41	announced	Updated project metadata.

Blattner M, Liu D, Robinson BD, Huang D, Poliakov A, Gao D, Nataraj S, Deonarine LD, Augello MA, Sailer V, Ponnala L, Ittmann M, Chinnaiyan AM, Sboner A, Chen Y, Rubin MA, Barbieri CE, Vivo through Coordinate Regulation of PI3K/mTOR and AR Signaling. Cancer Cell, 31(3):436-451(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: SPOP, prostate, cancer, mutation

Anton Poliakov
contact affiliation	Michigan Center for Translational Pathology, University of Michigan
contact email	poliakov@med.umich.edu
lab head
Anton Poliakov
contact affiliation	Pathology
contact email	poliakov@med.umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005309

PRIDE project URI

• PRIDE
  1. PXD005309
     1. Label: PRIDE project
     2. Name: SPOP mutation