PXD005308

PXD005308 is an original dataset announced via ProteomeXchange.

Title	Integrated Analysis of Global Proteome and Phosphoproteome in Cisplatin Resistant Bladder Cancer Cell Revealed the Molecular Signature Predictive to Patient Survival
Description	Cisplatin-based chemotherapy is the standard care regimen in bladder cancer (BC). However, resistance to the therapy whose molecular mechanisms of the resistance are not fully elucidated rapidly develops in BC patients. Here we introduced multidimensional proteomic analysis providing different levels of protein information, which included global proteome and phosphorpoteome perturbed by EGF using the cisplatin resistant BC model. Integrated analysis of protein expression and phosphorylation provided comprehensive profiles of altered proteins in cisplatin resistant cells that are dependent and independent on EGF, as well as suggesting significance of protein phosphorylation in resistance mechanisms in BC. From the reconstruction of cisplatin resistance associated network model and subsequent kinase enrichment analysis, we have identified three key kinases, CDK2, CHEK1, and ERBB2 as central regulators mediating cisplatin resistance. Experimental validation showed phosphorylation events in these central kinases and their putative substrates, suggesting evidence that activation of three kinases are important to acquired resistance to cisplatin in BC. Expanded analysis with this proteomic discovery to transcriptome profiles from BC cohorts nominated the 7 gene panel associated with poor survival after cisplatin-based chemotherapy. These findings provide insightful strategies to classify high-risk BC patients upon current chemotherapies and to identify therapeutic targets for recurrence disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:04:44.522.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jae Hun Jung
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-10 03:34:30	ID requested
1	2020-05-26 11:18:24	announced
⏵ 2	2024-10-22 05:04:45	announced	2024-10-22: Updated project metadata.

Jung JH, You S, Oh JW, Yoon J, Yeon A, Shahid M, Cho E, Sairam V, Park TD, Kim KP, Kim J, Integrated proteomic and phosphoproteomic analyses of cisplatin-sensitive and resistant bladder cancer cells reveal CDK2 network as a key therapeutic target. Cancer Lett, 437():1-12(2018) [pubmed]

10.1016/j.canlet.2018.08.014;

curator keyword: Biomedical

submitter keyword: Phosphoproteomics, bladder cancer, cisplatin resistance, biological network, molecular signature

Kwang Pyo Kim
contact affiliation	Kyung Hee University
contact email	kimkp@khu.ac.kr
lab head
Jae Hun Jung
contact affiliation	KHU
contact email	ultramanyo@naver.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/05/PXD005308

PRIDE project URI

• PRIDE
  1. PXD005308
     1. Label: PRIDE project
     2. Name: Integrated Analysis of Global Proteome and Phosphoproteome in Cisplatin Resistant Bladder Cancer Cell Revealed the Molecular Signature Predictive to Patient Survival