PXD005304

PXD005304 is an original dataset announced via ProteomeXchange.

Title	In-depth proteomics analysis of nicotinamide responses in triple negative breast cancer cells
Description	Triple negative breast cancer (TNBC) is characterized by an aggressive biologic behavior without specific targeted agent. Nicotinamide has recently been proven as a novel therapeutic agent for skin tumors in ONTRAC trial. Here we performed in-depth proteomes to characterize network of molecular interactions in TNBC cells treated by nicotinamide. Proteome profiles identified nicotinamide drives significant functional alterations related to major cellular pathways, including cell cycle, DNA replication, apoptosis and DNA damage repair. We further elaborated global signaling networks of molecular events with nicotinamide inducible expression changes in protein levels. This approach reveals that nicotinamide treatment rewires signaling networks towards dysfunction of DNA damage repair and away form a pro-growth state in TNBC. To our knowledge, our results of high resolution signaling network interactions provides the first evidence to comprehensively support the hypothesis of nicotinamide as a novel therapeutic agent in TNBC.
HostingRepository	PRIDE
AnnounceDate	2017-06-20
AnnouncementXML	Submission_2017-06-20_03:38:09.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dohyun Han
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-09 05:04:30	ID requested
⏵ 1	2017-06-20 03:38:10	announced

Kim JY, Lee H, Woo J, Yue W, Kim K, Choi S, Jang JJ, Kim Y, Park IA, Han D, Ryu HS, Reconstruction of pathway modification induced by nicotinamide using multi-omic network analyses in triple negative breast cancer. Sci Rep, 7(1):3466(2017) [pubmed]

curator keyword: Biomedical

submitter keyword: nicotinamide, TNBC, MDA-MB-231, TMT, quantitative proteomics

Dohyun Han
contact affiliation	Proteomics core facility, Seoul National University Hospital
contact email	hdh03@snu.ac.kr
lab head
Dohyun Han
contact affiliation	Colleage of medicine
contact email	hdh03@snu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005304
     1. Label: PRIDE project
     2. Name: In-depth proteomics analysis of nicotinamide responses in triple negative breast cancer cells