PXD005301

PXD005301 is an original dataset announced via ProteomeXchange.

Title	Quantitative phosphoproteomic analysis reveals system-wide signaling pathways regulated by site-specific phosphorylation on Keratin-8 in skin squamous cell carcinoma derived cell-line
Description	The epithelial specific keratin pair, Keratin 8/18 (K8/18), has been reported to be aberrantly expressed in squamous cell carcinoma (SCC) which is also correlated with increased invasiveness and poor prognosis. A Majority of Keratin 8 (K8) functions are governed by its phosphorylation at Serine73 (head domain) and Serine431 (tail domain) residues. Although deregulation of K8 phosphorylation has been associated with progression of different carcinomas, its role in skin SCC and the underlying mechanism is obscured. To understand the molecular basis of K8 phosphorylation mediated regulation of skin SCC progression, we performed TMT-based quantitative phosphoproteomics by expressing K8 wild type, phosphodead and phosphomimetic mutants in K8 deficient A431 cells. Bioinformatic analysis of our phosphoproteomic data showed differential regulation of phosphoproteins associated with migratory, proliferative and invasive potential in these cells. Further validation of protein phosphorylation levels and phenotypic validation of our phosphoproteomic data suggested potential role of K8 site specific phosphorylation/dephosphorylation in neoplastic progression of A431 cells.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:12.195.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Milind Vaidya
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue
Instrument	LTQ Orbitrap Velos; Q Exactive; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-09 03:27:55	ID requested
1	2018-07-05 05:55:15	announced
⏵ 2	2024-10-22 04:33:18	announced	2024-10-22: Updated project metadata.

10.1002/pmic.201600254;

Tiwari R, Sahu I, Soni BL, Sathe GJ, Datta KK, Thapa P, Sinha S, Vadivel CK, Dhaka B, Gowda H, Vaidya MM, Quantitative phosphoproteomic analysis reveals system-wide signaling pathways regulated by site-specific phosphorylation of Keratin-8 in skin squamous cell carcinoma derived cell line. Proteomics, 17(7):(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Keratin 8 phosphorylation, TMT based quantitative phosphoproteomics, Human epidermoid carcinoma cell-line

Milind M Vaidya
contact affiliation	Scientific Officer-G Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Navi Mumbai-410210, India
contact email	mvaidya@actrec.gov.in
lab head
Milind Vaidya
contact affiliation	Scientific Officer G
contact email	mvaidya@actrec.gov.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005301
     1. Label: PRIDE project
     2. Name: Quantitative phosphoproteomic analysis reveals system-wide signaling pathways regulated by site-specific phosphorylation on Keratin-8 in skin squamous cell carcinoma derived cell-line