PXD005299

PXD005299 is an original dataset announced via ProteomeXchange.

Title	Translating mRNAs strongly correlate to proteins in a multivariate manner and their translation ratios are phenotype specific
Description	As a well-known phenomenon, total mRNAs poorly correlate to proteins in their abundances as reported. Recent findings calculated with bivariate models suggested even poorer such correlation, whereas focusing on the translating mRNAs (ribosome nascent-chain complex-bound mRNAs, RNC-mRNAs) subset. In this study, we analysed the relative abundances of mRNAs, RNC-mRNAs and proteins on genome-wide scale, comparing human lung cancer A549 and H1299 cells with normal human bronchial epithelial (HBE) cells, respectively. As discovered, a strong correlation between RNC-mRNAs and proteins in their relative abundances could be established through a multivariate linear model by integrating the mRNA length as a key factor. The R2 reached 0.94 and 0.97 in A549 versus HBE and H1299 versus HBE comparisons, respectively. This correlation highlighted that the mRNA length significantly contributes to the translational modulation, especially to the translational initiation, favoured by its correlation with the mRNA translation ratio (TR) as observed. We found TR is highly phenotype specific, which was substantiated by both pathway analysis and biased TRs of the splice variants of BDP1 gene, which is a key transcription factor of transfer RNAs. These findings revealed, for the first time, the intrinsic and genome-wide translation modulations at translatomic level in human cells at steady-state, which are tightly correlated to the protein abundance and functionally relevant to cellular phenotypes.
HostingRepository	PRIDE
AnnounceDate	2022-02-25
AnnouncementXML	Submission_2022-02-25_04:57:35.450.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-09 03:08:32	ID requested
⏵ 1	2022-02-25 04:57:36	announced

Wang T, Cui Y, Jin J, Guo J, Wang G, Yin X, He QY, Zhang G, Translating mRNAs strongly correlate to proteins in a multivariate manner and their translation ratios are phenotype specific. Nucleic Acids Res, 41(9):4743-54(2013) [pubmed]

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project

curator keyword: Biological

submitter keyword: Translation ratios，Translating mRNAs

Qing-Yu He
contact affiliation	Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and 9 Technology, Jinan University, Guangzhou 510632, China
contact email	tqyhe@email.jnu.edu.cn
lab head
Yang Wang
contact affiliation	Jinan university
contact email	wangyang8857@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005299
     1. Label: PRIDE project
     2. Name: Translating mRNAs strongly correlate to proteins in a multivariate manner and their translation ratios are phenotype specific