PXD005291

PXD005291 is an original dataset announced via ProteomeXchange.

Title	A Hidden Human Proteome Encoded by the “Non-Coding” Genes
Description	It has been a long debate whether the 98% “non-coding” fraction of human genome can encode functional proteins besides a “random noise” of translation. We used our established translatome sequencing (RNC-seq) to analyze human cells and found that up to 3330 long non-coding RNAs (lncRNAs) were bound to ribosomes and thus might be translated into proteins (with more than 50 amino acids). These new protein-coding genes distributed universally in all human chromosomes. We then used various experimental methods including mass spectrometry, immunoblotting, subcellular localization and phenotype assessments to verify the existence of such a hidden human proteome encoded by purported lncRNAs that can express functional proteins. These new proteins deviate from the canonical proteins in various physical and chemical properties, and emerged mostly in primates during evolution. In sum, we experimentally evidenced a hidden human functional proteome encoded by purported lncRNAs, suggesting that the human genome has to be systematically re-annotated.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:33:50.411.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yang Wang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-08 03:47:06	ID requested
1	2022-02-25 04:50:11	announced
⏵ 2	2024-10-22 05:33:56	announced	2024-10-22: Updated project metadata.

Lu S, Zhang J, Lian X, Sun L, Meng K, Chen Y, Sun Z, Yin X, Li Y, Zhao J, Wang T, Zhang G, He QY, A hidden human proteome encoded by 'non-coding' genes. Nucleic Acids Res, 47(15):8111-8125(2019) [pubmed]

10.1093/nar/gkz646;

ProteomeXchange project tag: Chromosome-centric Human Proteome Project (C-HPP), Human Proteome Project

curator keyword: Biological

submitter keyword: New proteins，Translating lncRNAs

Qing-Yu He
contact affiliation	Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and 9 Technology, Jinan University, Guangzhou 510632, China
contact email	tqyhe@email.jnu.edu.cn
lab head
Yang Wang
contact affiliation	Jinan university
contact email	wangyang8857@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD005291

PRIDE project URI

• PRIDE
  1. PXD005291
     1. Label: PRIDE project
     2. Name: A Hidden Human Proteome Encoded by the “Non-Coding” Genes