PXD005287

PXD005287 is an original dataset announced via ProteomeXchange.

Title	Chemical cross-linking of the Spindly-RZZ complex followed by mass spectrometry
Description	Kinetochores are macromolecular assemblies that connect chromosomes to spindle microtubules during mitosis. The metazoan-specific ≈800 kilodalton ROD-Zwilch-ZW10 (RZZ) complex builds a fibrous corona that assembles on mitotic kinetochores prior to microtubule attachment to promote chromosome alignment and robust spindle assembly checkpoint signaling. Here, we combine biochemical reconstitutions, single particle electron cryo microscopy, cross-linking mass spectrometry, and structural modeling to build a complete model of human RZZ
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:34:05.588.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Victor Solis
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-07 10:10:56	ID requested
1	2022-02-28 03:28:57	announced
⏵ 2	2024-10-22 05:34:06	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

curator keyword: Biological

submitter keyword: human

Spindly-RZZ complex

Spindly

ROD

Zwilch

ZW10

CX-MS

orbitrap

Andrea Musacchio
contact affiliation	Director, Department of Mechanistic Cell Biology Max-Planck Institute of Molecular Physiology Dortmund
contact email	andrea.musacchio@mpi-dortmund.mpg.de
lab head
Victor Solis
contact affiliation	EpiQMAx GmbH
contact email	solis@genzentrum.lmu.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD005287

PRIDE project URI

• PRIDE
  1. PXD005287
     1. Label: PRIDE project
     2. Name: Chemical cross-linking of the Spindly-RZZ complex followed by mass spectrometry