PXD005286

PXD005286 is an original dataset announced via ProteomeXchange.

Title	Use of a modified Multiplexed Inhibitor Bead assay to study kinome responses to cytostatic treatment in human cancer cell lines
Description	In order to improve elution of proteins from the column, to reduce the cost as well as the hands on time of the analysis, we here present a modification of the Multiplexed Inhibitor Bead assay (MIB assay), where we elute the proteins from beads containing covalently bound inhibitors using trypsin digestion. Three human cancer cell lines treated with the same chemotherapeutics, cisplatin and docetaxel, were used to monitor and evaluate the performance of the assay. Using this method, we see a good coverage of the kinome as well as other important signaling proteins using only three kinase inhibitors. We demonstrate that the method is reproducible and that the column is re-usable for more than 10 times without loss in performance. The MIB assay revealed large differences in the cellular signaling responses between the cell lines.
HostingRepository	PRIDE
AnnounceDate	2017-02-13
AnnouncementXML	Submission_2017-02-13_03:40:29.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Hordeum vulgare (Barley); NCBI TaxID: 4513;
ModificationList	acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-07 09:43:37	ID requested
⏵ 1	2017-02-13 03:40:32	announced

Petrovic V, Olaisen C, Sharma A, Nepal A, Bugge S, Sundby E, Hoff BH, Slupphaug G, Otterlei M, On-column trypsinization allows for re-use of matrix in modified multiplexed inhibitor beads assay. Anal Biochem, 523():10-16(2017) [pubmed]

curator keyword: Technical, Biological

submitter keyword: kinome, MIB,

Voin Petrovic
contact affiliation	Department of Cancer Research and Molecular Medicine Department of Language and Literature Erling Skjalgssons g 1, aboratoriesenteret * * Laboratoriesenter Norwegian University of Science and Technology Trondheim 7030 Norway
contact email	voin.petrovic@ntnu.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/02/PXD005286

PRIDE project URI

• PRIDE
  1. PXD005286
     1. Label: PRIDE project
     2. Name: Use of a modified Multiplexed Inhibitor Bead assay to study kinome responses to cytostatic treatment in human cancer cell lines