PXD005285

PXD005285 is an original dataset announced via ProteomeXchange.

Title	SMAD2/3 interactome in human pluripotent stem cells
Description	This experiment was designed to determine the interactome of SMAD2/3 in human pluripotent stem cells (hPSCs). hPSCs were cultured in standard pluripotency-promoting conditions, or induced to differentiate towards the definitive endoderm lineage for 36h. Endogenous SMAD2/3 was immunoprecipitated from nuclear extracts in these two conditions using a specific antibody. Non-immune IgG immunoprecipitations were performed as negative controls. Three biological replicates per conditions were analyzed by quantitative label-free mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2018-01-04
AnnouncementXML	Submission_2019-02-12_08:11:52.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Bertero
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-07 09:23:07	ID requested
1	2018-01-04 02:28:20	announced
⏵ 2	2019-02-12 08:11:54	announced	Updated publication reference for PubMed record(s): 29489750.

Bertero A, Brown S, Madrigal P, Osnato A, Ortmann D, Yiangou L, Kadiwala J, Hubner NC, de Los Mozos IR, Sad, é, e C, Lenaerts AS, Nakanoh S, Grandy R, Farnell E, Ule J, Stunnenberg HG, Mendjan S, Vallier L, A mRNA methylation in pluripotency. Nature, 555(7695):256-259(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: SMAD2/3, human pluripotent stem cells

Ludovic Vallier
contact affiliation	Wellcome Trust - MRC Cambridge Stem Cell Institute Anne McLaren Laboratory and Department of Surgery, University of Cambridge, UK. Wellcome Trust Sanger Institute, Hinxton UK.
contact email	lv225@cam.ac.uk
lab head
Alessandro Bertero
contact affiliation	University of Washington
contact email	abertero@uw.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD005285

PRIDE project URI

• PRIDE
  1. PXD005285
     1. Label: PRIDE project
     2. Name: SMAD2/3 interactome in human pluripotent stem cells