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PXD005276

PXD005276 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleKinome profiling upon HCMV infection using the MIB-MS approach
DescriptionHuman cytomegalovirus (HCMV) is a significant cause of disease in immune-compromised adults and immune naïve newborns. No vaccine exists to prevent HCMV infection, and current antiviral therapies have toxic side effects that limit the duration and intensity of their use. There is thus an urgent need for new strategies to treat HCMV and repurposing existing drugs as antivirals is an attractive approach. Virus-induced changes in infected cells are often driven by changes in cellular kinase activity, leading us to hypothesize that defining the complement of kinases (the kinome), whose activity or expression is altered during infection would identify existing kinase inhibitors that could be repurposed as new antivirals. To this end, we applied a recently developed technique, MIB-MS kinome profiling, to quantitatively measure perturbations in >240 cellular kinases simultaneously in cells infected with a laboratory-adapted (AD169) or clinical (TB40E) HCMV strain using a label-free approach. Significant time-dependent changes for multiple kinases including cell cycle, receptor tyrosine and mitotic kinases were observed. Based on these data, we tested antiviral activity of 14 kinase inhibitors, and the most potent inhibitor blocked HCMV early gene expression and viral DNA accumulation. These results show the utility of kinome profiling to screen kinase inhibitors that can be repurposed as antivirals.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:33:51.510.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterLaura Herring
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-11-04 10:03:44ID requested
12017-03-20 07:17:12announced
22024-10-22 04:33:53announced2024-10-22: Updated project metadata.
Publication List
Arend KC, Lenarcic EM, Vincent HA, Rashid N, Lazear E, McDonald IM, Gilbert TS, East MP, Herring LE, Johnson GL, Graves LM, Moorman NJ, Kinome Profiling Identifies Druggable Targets for Novel Human Cytomegalovirus (HCMV) Antivirals. Mol Cell Proteomics, 16(4 suppl 1):S263-S276(2017) [pubmed]
10.1074/mcp.M116.065375;
Keyword List
submitter keyword: HCMV, human herpesvirus, kinase inhibitors,kinome, antivirals, virology, label-free quantitation
Contact List
Nathaniel J. Moorman
contact affiliationDepartment of Microbiology & Immunology UNC School of Medicine UNC-Chapel Hill
contact emailnathaniel_moorman@med.unc.edu
lab head
Laura Herring
contact affiliationUNC-Chapel Hill
contact emaillaura_herring@med.unc.edu
dataset submitter
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