PXD005276 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Kinome profiling upon HCMV infection using the MIB-MS approach |
Description | Human cytomegalovirus (HCMV) is a significant cause of disease in immune-compromised adults and immune naïve newborns. No vaccine exists to prevent HCMV infection, and current antiviral therapies have toxic side effects that limit the duration and intensity of their use. There is thus an urgent need for new strategies to treat HCMV and repurposing existing drugs as antivirals is an attractive approach. Virus-induced changes in infected cells are often driven by changes in cellular kinase activity, leading us to hypothesize that defining the complement of kinases (the kinome), whose activity or expression is altered during infection would identify existing kinase inhibitors that could be repurposed as new antivirals. To this end, we applied a recently developed technique, MIB-MS kinome profiling, to quantitatively measure perturbations in >240 cellular kinases simultaneously in cells infected with a laboratory-adapted (AD169) or clinical (TB40E) HCMV strain using a label-free approach. Significant time-dependent changes for multiple kinases including cell cycle, receptor tyrosine and mitotic kinases were observed. Based on these data, we tested antiviral activity of 14 kinase inhibitors, and the most potent inhibitor blocked HCMV early gene expression and viral DNA accumulation. These results show the utility of kinome profiling to screen kinase inhibitors that can be repurposed as antivirals. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:33:51.510.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Laura Herring |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-11-04 10:03:44 | ID requested | |
1 | 2017-03-20 07:17:12 | announced | |
⏵ 2 | 2024-10-22 04:33:53 | announced | 2024-10-22: Updated project metadata. |
Publication List
Arend KC, Lenarcic EM, Vincent HA, Rashid N, Lazear E, McDonald IM, Gilbert TS, East MP, Herring LE, Johnson GL, Graves LM, Moorman NJ, Kinome Profiling Identifies Druggable Targets for Novel Human Cytomegalovirus (HCMV) Antivirals. Mol Cell Proteomics, 16(4 suppl 1):S263-S276(2017) [pubmed] |
10.1074/mcp.M116.065375; |
Keyword List
submitter keyword: HCMV, human herpesvirus, kinase inhibitors,kinome, antivirals, virology, label-free quantitation |
Contact List
Nathaniel J. Moorman |
contact affiliation | Department of Microbiology & Immunology UNC School of Medicine UNC-Chapel Hill |
contact email | nathaniel_moorman@med.unc.edu |
lab head | |
Laura Herring |
contact affiliation | UNC-Chapel Hill |
contact email | laura_herring@med.unc.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005276
- Label: PRIDE project
- Name: Kinome profiling upon HCMV infection using the MIB-MS approach