PXD005274

PXD005274 is an original dataset announced via ProteomeXchange.

Title	Proteomic survey for search of biomarkers of colon tumors
Description	To the search of new colon tumor biomarkers in the transition from normal colon (NC) mucosa to adenoma (AD) and adenocarcinoma (AC), we integrated microarray data with the results of a high-throughput proteomic workflow. In proteomic study, we used a modified isoelectric focusing protocol on strips with an immobilized pH gradient to separate peptides labeled with iTRAQ (isobaric tags for relative and absolute quantitation) tags followed by liquid chromatography–tandem mass spectrometry analysis.
HostingRepository	PRIDE
AnnounceDate	2017-01-17
AnnouncementXML	Submission_2017-01-17_07:37:12.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD005274
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michał Kistowski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Phospho; iTRAQ4plex; Methylthio; Oxidation
Instrument	Q-Tof Premier

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-04 07:40:50	ID requested
1	2016-12-21 15:13:57	announced
⏵ 2	2017-01-17 07:37:15	announced	Updated project metadata.

Kistowski M, D, ę, bski J, Karczmarski J, Paziewska A, Ol, ę, dzki J, Mikula M, Ostrowski J, Dadlez M, A Strong Neutrophil Elastase Proteolytic Fingerprint Marks the Carcinoma Tumor Proteome. Mol Cell Proteomics, 16(2):213-227(2017) [pubmed]

Mikula M, Rubel T, Karczmarski J, Goryca K, Dadlez M, Ostrowski J, Integrating proteomic and transcriptomic high-throughput surveys for search of new biomarkers of colon tumors. Funct Integr Genomics, 11(2):215-24(2011) [pubmed]

curator keyword: Biomedical

submitter keyword: colon cancer, iTRAQ, isoelectric focusing, tubulo-villous adenoma, tubular adenoma, adenocarcinoma

Michał Dadlez
contact affiliation	Mass Spectrometry Laboratory, Institute of Biochemistry and Biophysics PAS
contact email	michald@ibb.waw.pl
lab head
Michał Kistowski
contact affiliation	Institute of Biochemistry and Biophysics PAS
contact email	mkisto@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD005274

PRIDE project URI

• PRIDE
  1. PXD005274
     1. Label: PRIDE project
     2. Name: Proteomic survey for search of biomarkers of colon tumors