PXD005273

PXD005273 is an original dataset announced via ProteomeXchange.

Title	STUDY OF THE PLASMA MEMBRANE PROTEOME DYNAMICS REVEALS NOVEL TARGETS OF THE NITROGEN REGULATION IN YEAST
Description	Yeast cells, to be able to grow on a wide variety of nitrogen sources, regulate the set of nitrogen transporters present at their plasma membrane. Such regulation relies on both transcriptional and post-translational events. While large-scale microarray studies have identified most nitrogen-sensitive genes, nitrogen-induced post-translational regulation has only been studied for very few proteins among which the general amino acid permease Gap1. Adding a preferred nitrogen source to proline-grown cells triggers Gap1 endocytosis and vacuolar degradation in an Rsp5-Bul1/2-dependent manner. Here, we used a large-scale proteomic approach to follow the dynamics of the plasma membrane proteome after addition of a preferred nitrogen source. We identified new targets of the nitrogen regulation and four transporters of poor nitrogen sources—Put4, Opt2, Dal5 and Ptr2—that rapidly decrease in abundance. Although the kinetics is different for each transporter, we found that three of them—Put4, Dal5 and Ptr2— are endocytosed, like Gap1, in an Rsp5-dependent manner and degraded in the vacuole. Finally, we showed that Gap1 stabilization at the plasma membrane, through deletion of Bul proteins, regulates the abundance of Put4, Dal5 and Ptr2.
HostingRepository	PRIDE
AnnounceDate	2017-07-10
AnnouncementXML	Submission_2017-07-10_00:30:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hervé Degand
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-04 05:13:26	ID requested
⏵ 1	2017-07-10 00:30:05	announced

Villers J, Savocco J, Szopinska A, Degand H, Nootens S, Morsomme P, Study of the Plasma Membrane Proteome Dynamics Reveals Novel Targets of the Nitrogen Regulation in Yeast. Mol Cell Proteomics, 16(9):1652-1668(2017) [pubmed]

submitter keyword: yeast plasma membrane, proteomics, nitrogen sources, transporters, endocytosis, arrestin

Pierre Morsomme
contact affiliation	Université catholique de Louvain, Institut des Sciences de la Vie, Croix du Sud 4-5, B-1348 Louvain-la-Neuve
contact email	pierre.morsomme@uclouvain.be
lab head
Hervé Degand
contact affiliation	Université Catholique de Louvain La Neuve
contact email	herve.degand@uclouvain.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005273
     1. Label: PRIDE project
     2. Name: STUDY OF THE PLASMA MEMBRANE PROTEOME DYNAMICS REVEALS NOVEL TARGETS OF THE NITROGEN REGULATION IN YEAST