PXD005271

PXD005271 is an original dataset announced via ProteomeXchange.

Title	Proteome dynamics during postnatal mouse corpus callosum development
Description	Formation of cortical connections requires the precise coordination of several discrete stages. This is particularly significant with regard to the corpus callosum, the largest white matter structure bridging both cerebral hemispheres, whose development undergoes several dynamic stages including the crossing of axon projections, the elimination of exuberant projections, and the myelination of established tracts. To comprehensively characterize the molecular events in this dynamic process, we set to determine the distinct temporal expression of proteins regulating the formation of the corpus callosum and their respective developmental functions. Mass spectrometry-based proteomic profiling was performed on early postnatal mouse corpus callosi, for which limited evidence has been obtained previously, using stable isotope of labeled amino acids in mammals (SILAM). The analyzed corpus callosi had distinct proteomic profiles depending on age, indicating rapid progression of specific molecular events during this period. The proteomic expression profiles were then segregated into five separate protein clusters, each with distinct trajectories relevant to their intended developmental functions. Our analysis both confirms many previously-identified proteins in aspects of corpus callosum development, and identifies new candidates in understudied areas of development including callosal axon refinement. We present a valuable resource for identifying new proteins integral to corpus callosum development that will provide new insights into the development and diseases afflicting this structure.
HostingRepository	PRIDE
AnnounceDate	2017-03-29
AnnouncementXML	Submission_2017-04-04_02:16:05.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexander Son
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-04 04:37:08	ID requested
1	2017-03-29 06:23:23	announced
⏵ 2	2017-04-04 02:16:07	announced	Updated publication reference for PubMed record(s): 28349996.

Son AI, Fu X, Suto F, Liu JS, Hashimoto-Torii K, Torii M, Proteome dynamics during postnatal mouse corpus callosum development. Sci Rep, 7():45359(2017) [pubmed]

curator keyword: Biological

submitter keyword: Corpus callosum, axon, glia, refinement, axon guidance

Masaaki Torii
contact affiliation	Center for Neuroscience Research Children's National Medical Center 111 Michigan Avenue N.W., M7631 Washington, DC 20010-2970 Phone: +1-202-476-4279 Fax: +1-202-476-4988 e-mail: MTorii@childrensnational.org
contact email	mtorii@childrensnational.org
lab head
Alexander Son
contact affiliation	Children's National Medical System
contact email	ason@childrensnational.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/03/PXD005271

PRIDE project URI

• PRIDE
  1. PXD005271
     1. Label: PRIDE project
     2. Name: Proteome dynamics during postnatal mouse corpus callosum development