PXD005266

PXD005266 is an original dataset announced via ProteomeXchange.

Title	Proteasome activity profiling uncovers alteration of catalytic β2 and β5 subunits of the stress-induced proteasome during salinity stress in tomato roots
Description	The stress proteasome in the animal kingdom facilitates faster conversion of oxidized proteins during stress conditions by incorporating different catalytic β subunits. Plants deal with similar kind of stresses and also carry multiple paralogous genes encoding for each of the three catalytic β subunits. Here we investigated the existence of stress proteasomes upon salt stress in tomato roots. In contrast to Arabidopsis thaliana, tomato has a simplified proteasome gene set with single genes encoding each β subunit except for two genes encoding β2. Using proteasome activity profiling on tomato roots during salt stress we discovered a transient modification of the catalytic subunits of the proteasome coinciding with a loss of viability. This stress-induced proteasome disappears at later time points and coincides with the need to degrade oxidized proteins during salt stress. Subunit-selective proteasome probes and MS analysis of fluorescent 2D gels demonstrated that the stress-induced proteasome is not caused by altered subunit assembly, but involves an increased molecular weight (MW) of both β2 and β5 subunits, and an additional acidic pI shift for β5, whilst β1 remains mostly unchanged. Although the underlying subunit modification is unknown, we can rule out ubiquitination, phosphorylation and glycosylation as underlying molecular mechanisms. This stress-induced proteasome may play an important role in PCD during abiotic stress.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:05.050.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Farnusch Kaschani
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-04 00:42:25	ID requested
1	2017-02-22 02:02:28	announced
⏵ 2	2024-10-22 04:33:05	announced	2024-10-22: Updated project metadata.

10.3389/fpls.2017.00107;

Kov, á, cs J, Po, ó, r P, Kaschani F, Chandrasekar B, Hong TN, Misas-Villamil JC, Xin BT, Kaiser M, Overkleeft HS, Tari I, van der Hoorn RA, 5 Subunits of the Stress-Induced Proteasome during Salinity Stress in Tomato Roots. Front Plant Sci, 8():107(2017) [pubmed]

curator keyword: Biological

submitter keyword: tomato root, programmed cell death, salt stress, catalytic subunit, immune proteasome,20S proteasome, activity-based protein profiling

Farnusch Kaschani
contact affiliation	Universität Duisburg-Essen ZMB, Chemische Biologie, AG Kaiser Universitätsstr. 2 45117 Essen
contact email	farnusch.kaschani@uni-due.de
lab head
Farnusch Kaschani
contact affiliation	University Duisburg-Essen
contact email	farnusch.kaschani@uni-due.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005266
     1. Label: PRIDE project
     2. Name: Proteasome activity profiling uncovers alteration of catalytic β2 and β5 subunits of the stress-induced proteasome during salinity stress in tomato roots