PXD005259

PXD005259 is an original dataset announced via ProteomeXchange.

Title	Proteomics of Diverse Clonal Groups of Enterotoxigenic Escherichia coli Underline Variations in Surface Antigens and Similarities in Metabolism
Description	Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrhoea, a pervasive disease in low- and middle-income countries that affects mostly young children and visiting travellers. Substantial diversity exists among ETEC isolates, hindering development of effective preventive means. To investigate how ETEC genomic variation is reflected at expressed proteome level, we applied label-free quantitative proteomics to 7 human ETEC strains representing 5 epidemiologically important lineages. We further determined the protein profile of the non-pathogenic E. coli B strain BL21 to discriminate metabolic features specific for ETEC. The analysis yielded a dataset of 2,894 proteins, of which 1,777 were present in all strains. Each ETEC strain displayed on average 27 (±10) proteins with known or putative links to virulence, both plasmid- and chromosomally-encoded, and a number of strain-specific isoforms participating in the biosynthesis of surface antigens. Statistical comparison of relative protein levels between the ETEC strains and BL21 revealed several proteins with significantly increased amounts only in BL21, including enzymes of arginine biosynthesis and metabolism of melibiose, galactitol and gluconate. ETEC strains displayed consistently increased levels of proteins that were functional in iron acquisition, maltose metabolism, and acid resistance. These results suggest that specific metabolic functions are shared among ETEC isolates.
HostingRepository	PRIDE
AnnounceDate	2017-12-06
AnnouncementXML	Submission_2017-12-06_09:14:43.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Veronika Kucharova
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-02 04:23:51	ID requested
⏵ 1	2017-12-06 09:14:44	announced

Pettersen VK, Steinsland H, Wiker HG, Comparative Proteomics of Enterotoxigenic Escherichia coli Reveals Differences in Surface Protein Production and Similarities in Metabolism. J Proteome Res, 17(1):325-336(2018) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Enterotoxigenic Escherichia coli, Label-Free Quantification, Proteogenomics, Bacterial Metabolism, Plasmid, Virulence, H10407

Harald G. Wiker
contact affiliation	The Gade Research Group for Infection and Immunity, Department of Clinical Science, University of Bergen, Norway
contact email	Harald.Wiker@uib.no
lab head
Veronika Kucharova
contact affiliation	University of Bergen
contact email	veronika.kucharova@k2.uib.no
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD005259

PRIDE project URI

• PRIDE
  1. PXD005259
     1. Label: PRIDE project
     2. Name: Proteomics of Diverse Clonal Groups of Enterotoxigenic Escherichia coli Underline Variations in Surface Antigens and Similarities in Metabolism