PXD005257

PXD005257 is an original dataset announced via ProteomeXchange.

Title	Chlamydomonas reinhardtii quantification of Mannosidase 1A and Xylosyltransferase1A
Description	We have identified insertional mutants of mannosidase 1A and xylosyltransferase 1A in Chlamydomonas reinhardtii. To verify a knockout/knockdown of the enzymes in the insertional mutant strains, PRM measurements were performed. Mass spectrometric analyses of intact N-glycopeptides revealed that disruption of the genes altered the N-glycan composition of the alga (in single as well as double mutant).
HostingRepository	PRIDE
AnnounceDate	2018-02-27
AnnouncementXML	Submission_2018-02-27_05:50:38.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Schulze
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-02 03:58:21	ID requested
⏵ 1	2018-02-27 05:50:39	announced

Schulze S, Oltmanns A, Machnik N, Liu G, Xu N, Jarmatz N, Scholz M, Sugimoto K, Fufezan C, Huang K, Hippler M, . Plant Physiol, 176(3):1952-1964(2018) [pubmed]

curator keyword: Biological

submitter keyword: Chlamydomonas reinhardtii, N-glycosylation, quantification

Michael Hippler
contact affiliation	Institute of Plant Biology and Biotechnology, University of Muenster
contact email	mhippler@uni-muenster.de
lab head
Stefan Schulze
contact affiliation	University of Muenster
contact email	stefan.schulze@uni-muenster.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD005257
     1. Label: PRIDE project
     2. Name: Chlamydomonas reinhardtii quantification of Mannosidase 1A and Xylosyltransferase1A