PXD005256

PXD005256 is an original dataset announced via ProteomeXchange.

Title	Quantitative analysis of differential proteome expression in wild-type and mutants strains of Salmonella enterica serovar Typhimurium
Description	The ability to adapt quickly to changing environmental conditions is crucial for growth and survival of bacteria in their natural environment. The common strategy that bacteria utilize to increase their survival under stressful conditions, including antibiotic treatment, is the entry into a non-actively growing state (quiescence). In the wide host-range pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) and other Gram negative bacteria, this temporary arrest of proliferation induces the expression of the alternative sigma subunit σS/RpoS of RNA polymerase (RNAP) which remodels global gene expression to reshape the cell physiology and ensure survival in various stress conditions and nutritional deficiencies (i.e. general stress resistance) and long-term cell persistence. σS is also required for virulence and biofilm formation. Our work aims at understanding key molecular and physiological determinants responsible for persistence of non-growing bacteria, using the ubiquitous pathogen S. Typhimurium as a working system. We expect to identify novel functional proteins that are both physiologically important and of wide biological significance. One approach to characterize those proteins is to compare mutant proteome to that of the wild-type strain to unravel potential effects of the mutations on the expression and/or stability of proteins.
HostingRepository	PRIDE
AnnounceDate	2018-10-26
AnnouncementXML	Submission_2018-10-26_13:18:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Thibaut Douché
SpeciesList	scientific name: Salmonella enterica subsp. enterica serovar Typhimurium str. 14028S; NCBI TaxID: 588858;
ModificationList	L-methionine sulfoxide; N-acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-02 03:42:00	ID requested
⏵ 1	2018-10-26 13:18:27	announced

Lago M, Monteil V, Douche T, Guglielmini J, Criscuolo A, Maufrais C, Matondo M, Norel F, in Salmonella: identification of small proteins and evidence for post-transcriptional regulation. Sci Rep, 7(1):2127(2017) [pubmed]

submitter keyword: Salmonella enterica serovar Typhimurium, shotgun proteomics, Q Exactive HF, MaxQuant, Perseus

Françoise Norel
contact affiliation	Department of microbiology, Macromolecular systems and signaling Lab, Persistence of non-proliferating bacteria Group, Institut Pasteur, Paris, France. ( lab head )
contact email	francoise.norel@pasteur.fr
lab head
Thibaut Douché
contact affiliation	Institut Pasteur
contact email	thibaut.douche@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD005256

PRIDE project URI

• PRIDE
  1. PXD005256
     1. Label: PRIDE project
     2. Name: Quantitative analysis of differential proteome expression in wild-type and mutants strains of Salmonella enterica serovar Typhimurium