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PXD005255

PXD005255 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBacteroides fragilis outer membrane vesicles
DescriptionNumber of publications show that intestinal microflora vesicles secretion is essential for the maintaining homeostasis. Conducted comprehensive genomic, transcriptomic and proteomic studies of vesicles determine the nature and the basic mechanisms of bacterial influence to the host. The role of the vesicles to a greater extent is defined as carriers of virulence factors or enzymes determining the nutritional function. In addition some bacterial vesicles have a special mission. B.fragilis vesicles are providing anti-inflammatory effect on the colon immune cells. However there is no information about possible role of vesicles secreted by ETBF, contributing bowel disease development and colon cancer. To determine possible effects of ETBF vesicles to the host compared with NTBF, we performed comprehensive proteome and for the first time metabolome analysis and reconstructed pathways for vesicles of both strains with subsequent confirmation of the activity of one of the main pathway by fluxome analysis. A detailed analysis of the vesicles allowed us to discover a new quality of ETBF vesicles as intelligent systems capable of absorbing and processing of exogenous substrates. We believe that this property allows them to maintain stability for a long time and implement mechanisms of pathogenicity.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:33:46.382.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD005255
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterIvan Butenko
SpeciesList scientific name: Bacteroides fragilis (strain ATCC 25285 / NCTC 9343); NCBI TaxID: 272559;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-11-02 03:21:38ID requested
12017-11-13 00:28:37announced
22024-10-22 04:33:48announced2024-10-22: Updated project metadata.
Publication List
Zakharzhevskaya NB, Vanyushkina AA, Altukhov IA, Shavarda AL, Butenko IO, Rakitina DV, Nikitina AS, Manolov AI, Egorova AN, Kulikov EE, Vishnyakov IE, Fisunov GY, Govorun VM, Outer membrane vesicles secreted by pathogenic and nonpathogenic Bacteroides fragilis represent different metabolic activities. Sci Rep, 7(1):5008(2017) [pubmed]
10.6019/PXD005255;
10.1038/s41598-017-05264-6;
Keyword List
curator keyword: Biological, Biomedical
submitter keyword: proteomics,Bacteroides fragilis, vesicles
Contact List
Gleb Fisunov
contact affiliationLaboratory of proteomic analysis of Scientific Research Institute of Physical-Chemical Medicine, Moscow, Russia
contact emailherr.romanoff@gmail.com
lab head
Ivan Butenko
contact affiliationScientific Research Institute for Systems Biology and Medicine, Moscow, Russian Federation
contact emailivan.butenko@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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