PXD005251 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Architecture of the yeast Elongator complex |
Description | The highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1-6) that are all equally important for its tRNA modification activity. However, its overall architecture and the detailed reaction mechanism remain elusive. Here, we report the structures of the fully assembled yeast Elongator and the Elp123 sub-complex solved by an integrative structure determination approach showing that two copies of the Elp1, Elp2 and Elp3 subunits form a two-lobed scaffold, which binds Elp456 asymmetrically. Our topological models are consistent with previous studies on individual subunits and further validated by complementary biochemical analyses. Our study provides a structural framework on how the tRNA modification activity is carried out by Elongator. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:33:45.269.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Alessandro Ori |
SpeciesList | scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932; |
ModificationList | iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2016-11-02 02:28:49 | ID requested | |
1 | 2016-12-20 12:40:50 | announced | |
2 | 2017-10-24 03:09:36 | announced | Updated project metadata. |
⏵ 3 | 2024-10-22 04:33:51 | announced | 2024-10-22: Updated project metadata. |
Publication List
Dauden MI, Kosinski J, Kolaj-Robin O, Desfosses A, Ori A, Faux C, Hoffmann NA, Onuma OF, Breunig KD, Beck M, Sachse C, S, é, raphin B, Glatt S, M, ü, ller CW, Architecture of the yeast Elongator complex. EMBO Rep, 18(2):264-279(2017) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: tRNA modification,Elongator, yeast, electron microscopy, Saccharomyces cerevisiae |
Contact List
Martin Beck |
contact affiliation | Structural and Computational Biology Unit European Molecular Biology Laboratory Meyerhofstr. 1 69117 Heidelberg Germany |
contact email | martin.beck@embl.de |
lab head | |
Alessandro Ori |
contact affiliation | Leibniz Institute on Aging |
contact email | aleori@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD005251
- Label: PRIDE project
- Name: Architecture of the yeast Elongator complex