PXD005251

PXD005251 is an original dataset announced via ProteomeXchange.

Title	Architecture of the yeast Elongator complex
Description	The highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1-6) that are all equally important for its tRNA modification activity. However, its overall architecture and the detailed reaction mechanism remain elusive. Here, we report the structures of the fully assembled yeast Elongator and the Elp123 sub-complex solved by an integrative structure determination approach showing that two copies of the Elp1, Elp2 and Elp3 subunits form a two-lobed scaffold, which binds Elp456 asymmetrically. Our topological models are consistent with previous studies on individual subunits and further validated by complementary biochemical analyses. Our study provides a structural framework on how the tRNA modification activity is carried out by Elongator.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:45.269.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alessandro Ori
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2016-11-02 02:28:49	ID requested
1	2016-12-20 12:40:50	announced
2	2017-10-24 03:09:36	announced	Updated project metadata.
⏵ 3	2024-10-22 04:33:51	announced	2024-10-22: Updated project metadata.

Dauden MI, Kosinski J, Kolaj-Robin O, Desfosses A, Ori A, Faux C, Hoffmann NA, Onuma OF, Breunig KD, Beck M, Sachse C, S, é, raphin B, Glatt S, M, ü, ller CW, Architecture of the yeast Elongator complex. EMBO Rep, 18(2):264-279(2017) [pubmed]

curator keyword: Biological

submitter keyword: tRNA modification,Elongator, yeast, electron microscopy, Saccharomyces cerevisiae

Martin Beck
contact affiliation	Structural and Computational Biology Unit European Molecular Biology Laboratory Meyerhofstr. 1 69117 Heidelberg Germany
contact email	martin.beck@embl.de
lab head
Alessandro Ori
contact affiliation	Leibniz Institute on Aging
contact email	aleori@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD005251

PRIDE project URI

• PRIDE
  1. PXD005251
     1. Label: PRIDE project
     2. Name: Architecture of the yeast Elongator complex