⮝ Full datasets listing

PXD005251

PXD005251 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleArchitecture of the yeast Elongator complex
DescriptionThe highly conserved eukaryotic Elongator complex performs specific chemical modifications on wobble base uridines of tRNAs, which are essential for proteome stability and homeostasis. The complex is formed by six individual subunits (Elp1-6) that are all equally important for its tRNA modification activity. However, its overall architecture and the detailed reaction mechanism remain elusive. Here, we report the structures of the fully assembled yeast Elongator and the Elp123 sub-complex solved by an integrative structure determination approach showing that two copies of the Elp1, Elp2 and Elp3 subunits form a two-lobed scaffold, which binds Elp456 asymmetrically. Our topological models are consistent with previous studies on individual subunits and further validated by complementary biochemical analyses. Our study provides a structural framework on how the tRNA modification activity is carried out by Elongator.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:33:45.269.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAlessandro Ori
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListiodoacetamide derivatized residue
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02016-11-02 02:28:49ID requested
12016-12-20 12:40:50announced
22017-10-24 03:09:36announcedUpdated project metadata.
32024-10-22 04:33:51announced2024-10-22: Updated project metadata.
Publication List
Dauden MI, Kosinski J, Kolaj-Robin O, Desfosses A, Ori A, Faux C, Hoffmann NA, Onuma OF, Breunig KD, Beck M, Sachse C, S, é, raphin B, Glatt S, M, ü, ller CW, Architecture of the yeast Elongator complex. EMBO Rep, 18(2):264-279(2017) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: tRNA modification,Elongator, yeast, electron microscopy, Saccharomyces cerevisiae
Contact List
Martin Beck
contact affiliationStructural and Computational Biology Unit European Molecular Biology Laboratory Meyerhofstr. 1 69117 Heidelberg Germany
contact emailmartin.beck@embl.de
lab head
Alessandro Ori
contact affiliationLeibniz Institute on Aging
contact emailaleori@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/12/PXD005251
PRIDE project URI
Repository Record List
[ + ]